Diabetes, Vol 29, Issue 11 861-864, Copyright © 1980 by American Diabetes Association

ARTICLES

Red cell sorbitol: an indicator of diabetic control

JI Malone, G Knox, S Benford and TA Tedesco

Intact human erythrocytes accumulate intracellular sorbitol in response to the medium's glucose concentration during in vitro incubations. Sorbitol was identified and measured both enzymatically and by gas-liquid chromatography. The sorbitol produced is most likely a result of the activity of aldose reductase, since (1) a low glucose concentration in the medium elicits this response, and (2) this activity is completely blocked by tetramethylene glutaric acid, a specific inhibitor of aldose reductase. Erythrocyte sorbitol levels in insulin-dependent diabetics are clearly above those of nondiabetics after an 8 h fast. A good correlation exists between red cell sorbitol content and coincident plasma glucose concentrations. Individual exceptions to this rule exist, however, and suggest that red cell sorbitol levels may provide information about in vivo polyol pathway activity that may be important in the pathogenesis of diabetes-associated complications.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Kaiserova, S. Srivastava, J. D. Hoetker, S. O. Awe, X.-L. Tang, J. Cai, and A. Bhatnagar Redox Activation of Aldose Reductase in the Ischemic Heart J. Biol. Chem., June 2, 2006; 281(22): 15110 - 15120. [Abstract] [Full Text] [PDF]

				Z. Dagher, Y. S. Park, V. Asnaghi, T. Hoehn, C. Gerhardinger, and M. Lorenzi Studies of Rat and Human Retinas Predict a Role for the Polyol Pathway in Human Diabetic Retinopathy Diabetes, September 1, 2004; 53(9): 2404 - 2411. [Abstract] [Full Text] [PDF]

				A. S. Galvez, J. A. Ulloa, M. Chiong, A. Criollo, V. Eisner, L. F. Barros, and S. Lavandero Aldose Reductase Induced by Hyperosmotic Stress Mediates Cardiomyocyte Apoptosis: DIFFERENTIAL EFFECTS OF SORBITOL AND MANNITOL J. Biol. Chem., October 3, 2003; 278(40): 38484 - 38494. [Abstract] [Full Text] [PDF]

				V. Asnaghi, C. Gerhardinger, T. Hoehn, A. Adeboje, and M. Lorenzi A Role for the Polyol Pathway in the Early Neuroretinal Apoptosis and Glial Changes Induced by Diabetes in the Rat Diabetes, February 1, 2003; 52(2): 506 - 511. [Abstract] [Full Text] [PDF]

				W. R. Tracey, W. P. Magee, C. A. Ellery, J. T. MacAndrew, A. H. Smith, D. R. Knight, and P. J. Oates Aldose reductase inhibition alone or combined with an adenosine A3 agonist reduces ischemic myocardial injury Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1447 - H1452. [Abstract] [Full Text] [PDF]

				R. Ramasamy, N. Trueblood, and S. Schaefer Metabolic effects of aldose reductase inhibition during low-flow ischemia and reperfusion Am J Physiol Heart Circ Physiol, July 1, 1998; 275(1): H195 - H203. [Abstract] [Full Text] [PDF]

				S. Gupta, E. Chough, J. Daley, P. Oates, K. Tornheim, N. B. Ruderman, and J. F. Keaney Jr. Hyperglycemia increases endothelial superoxide that impairs smooth muscle cell Na+-K+-ATPase activity Am J Physiol Cell Physiol, March 1, 2002; 282(3): C560 - C566. [Abstract] [Full Text] [PDF]