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Diabetes, Vol 29, Issue 12 1044-1047, Copyright © 1980 by American Diabetes Association

ARTICLES

Measurement of glycosylated amino acids and peptides from urine of diabetic patients using affinity chromatography

M Brownlee, H Vlassara and A Cerami

An affinity chromatography system has been developed that retains glycosylated amino acids and peptides. Using this system, synthetic 14C-glycosylated lysine (reduced with NaB3H4) was completely separated from a mixture of reduced 14C-glycosylated lysine and unmodified 3H-lysine. Amino acid analysis of the retained peak from hydrolyzed human diabetic hemoglobin previously reduced with NaB3H4 revealed an equimolar mixture of glycosylated valine and glycosylated lysine, in agreement with previously published data obtained using other methodologies. These data demonstrate that in alkaline solution, the NaB3H4-reducible breakdown products of nonenzymatically glycosylated proteins are adsorbed to m-aminophenyl boronic acid immobilized on Bio-Gel P-6, while nonglycosylated amino acids are not. This affinity chromatography system should facilitate the rapid evaluation of nonenzymatic glycosylation in a large number of diabetic tissues. Levels of retained compounds in urine from diabetic and normal patients were determined by measuring ninhydrin-positive material. Amino acid analysis of NaB3H4-reduced hydrolysates of these peaks showed that glycosylated lysine was the major borohydride-reducible adduct present (67%--86%). Linear regression analysis showed that the quantity of excreted compounds in normals correlated with body weight (r = 0.63). The mean level (mumol leucine-equivalent/24 h/kg body weight) in diabetics was over 1.5 times that found in urine from normal subjects (P < 0.005).
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Copyright © 1980 by the American Diabetes Association.