Diabetes, Vol 29, Issue 2 151-155, Copyright © 1980 by American Diabetes Association

ARTICLES

Defective regulation of Cl- permeability in islets of diabetic mice [C57BL/KsJ(db/db)]

O Berglund and J Sehlin

Efflux of 36Cl- from prelabeled, collagenase-isolated islets of noninbred ob/ob mice, inbred diabetic [C57BL/KsJ(db/db)] mice, and nondiabetic [C57BL/KsJ(+/+)] mice was studied by nonrecirculating perifusion. Islets of both ob/ob mice and nondiabetic KsJ mice showed similar rates of basal 36Cl- efflux, D-glucose stimulation of the 36Cl- efflux, and net uptake of 36Cl- at apparent isotope equilibrium. The 36Cl- efflux in islets from both young and old KsJ-db/db mice was almost insensitive to the D-glucose concentration. The basal rate of 36Cl- efflux in islets from young and old db/db mice was increased, indicating an abnormally high Cl- permeability. It is suggested that the defective regulation of the membrane potential in B-cells from [C57BL/KsJ(db/db)] mice may at least partly be caused by a db-mediated defect in the regulation of Cl- permeability.
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