Diabetes, Vol 29, Issue 6 497-500, Copyright © 1980 by American Diabetes Association
Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts
P Meda, EL Hooghe-Peters and L Orci
A simple method that permits the attachment of fully dissociated adult
pancreatic islet cells to culture dishes, using osmotically disrupted
fibroblasts, is described. Using this system, monolayer cultures consisting
of either isolated or clustered adult endocrine islet cells can be readily
established in standard culture media.