Diabetes, Vol 31, Issue 12 1077-1083, Copyright © 1982 by American Diabetes Association

ARTICLES

Binding, internalization, and degradation of insulin in vascular endothelial cells

N Kaiser, I Vlodavsky, A Tur-Sinai, Z Fuks and E Cerasi

The interaction of insulin with the vascular endothelium was studied using bovine aortic endothelial cells in monolayer cultures. Confluent cell cultures exhibited specific binding of 125I-insulin to high- and low-affinity cell surface receptor sites. Binding was reversible, saturable, and accompanied by internalization and degradation of the bound hormone in a temperature- and time-dependent manner. Pre-exposure of the cultures to insulin resulted in a time-dependent reduction in the availability of cell surface receptors (downregulation). It is concluded that the occurrence of reversible insulin binding and of insulin degradation in endothelial cells supports the concept that the vascular endothelium compartment may regulate the level of insulin in the circulation.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M Bendayan and E. Rasio Transport of insulin and albumin by the microvascular endothelium of the rete mirabile J. Cell Sci., January 7, 1996; 109(7): 1857 - 1864. [Abstract] [PDF]

				S.-Z. Han, Y. Ouchi, H. Karaki, and H. Orimo Inhibitory Effects of Insulin on Cytosolic Ca2+ Level and Contraction in the Rat Aorta : Endothelium-Dependent and -Independent Mechanisms Circ. Res., October 1, 1995; 77(4): 673 - 678. [Abstract] [Full Text]

				G. King and S. Johnson Receptor-mediated transport of insulin across endothelial cells Science, March 29, 1985; 227(4694): 1583 - 1586. [Abstract] [PDF]