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Diabetes, Vol 31, Issue 3 238-241, Copyright © 1982 by American Diabetes Association

ARTICLES

Preservation of morphology, insulin biosynthesis, and insulin release in cryopreserved human fetal pancreas

S Sandler, A Andersson, C Hellerstrom, B Petersson, I Swenne, C Bjorken and CG Groth

Human fetal pancreatic glands were obtained from 12 consecutive prostaglandin-induced abortions. Explants cultured for 1 day were frozen at 0.3 degree C/min in a 1 M DMSO-containing medium and stored at -196 degrees C. After storage for 3-4 mo the frozen material was rapidly thawed and cultured 1 day before being tested for functional performance. There was a positive correlation between the pancreatic insulin content and the fetal crown-heel length. Seven of the twelve fetuses showed a marked insulin response to an acute glucose-theophylline challenge. In five of these pancreases there was a well-preserved morphology after thawing, whereas only one of the nonresponding preparations showed a satisfactory morphology. Pancreatic explants from three of four fetuses tested displayed evidences of an (pro)insulin biosynthesis. The combined results indicated that low-temperature storage of human fetal endocrine pancreas is compatible with specific functional survival.
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Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 1982 by the American Diabetes Association.