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Diabetes, Vol 31, Issue 7 656-658, Copyright © 1982 by American Diabetes Association

ARTICLES

Cell-free synthesis of rat insulin-like growth factor II

AM Acquaviva, CB Bruni, SP Nissley and MM Rechler

Total RNA extracted from a rat liver cell line (BRL 3A) that synthesizes rat insulin-like growth factor II (IGF-II) was translated in a reticulocyte lysate cell-free system. Incubation of the translation products with antisera to mature rat IGF-II (Mr 8700) selectively immunoprecipitated a Mr 21,600 protein. We propose that this protein represents pre-pro-IGF-II.
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Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 1982 by the American Diabetes Association.