Diabetes, Vol 34, Issue 9 850-854, Copyright © 1985 by American Diabetes Association

ARTICLES

Biohybrid artificial pancreas. Long-term insulin secretion by devices seeded with canine islets

Y Araki, BA Solomon, RM Basile and WL Chick

Long-term insulin secretion was investigated in seven biohybrid capillary devices seeded with canine islets. Approximately 50,000 islets could be isolated from a single canine pancreas using collagenase digestion in conjunction with the recently described Velcro technique. Devices were perfused with tissue culture medium 199 containing 300 mg/dl glucose. Insulin secretion fell during the first 1-2 days of culture from approximately 18 to 6 U/day. After 3-4 wk of perfusion, however, there was a gradual rise in insulin output that reached greater than 15 U/day after 7 wk. Insulin output eventually stabilized at 18-20 U/day. Two of these devices were studied for 80 and 86 days, respectively, and continued to secrete these same amounts of insulin. Glucose-induced insulin release was studied after 1 and 4 wk of culture and was well preserved.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?