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Diabetes, Vol 35, Issue 6 649-653, Copyright © 1986 by American Diabetes Association

ARTICLES

A method for the mass isolation of islets from the adult pig pancreas

C Ricordi, EH Finke and PE Lacy

A method for mass isolation of islets from the pig pancreas is described. The procedure involves the use of an enzymatic and mechanical pancreatic digestion procedure followed by filtration and separation of the islets on Ficoll gradients. A remarkably high yield of purified islets has been obtained from the pig pancreas with this procedure. The islets are morphologically intact and respond to acute stimulation with glucose in vitro.
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Copyright © 1986 by the American Diabetes Association.