Diabetes, Vol 35, Issue 6 668-674, Copyright © 1986 by American Diabetes Association

ARTICLES

Acetone metabolism in humans during diabetic ketoacidosis

GA Reichard, CL Skutches, RD Hoeldtke and OE Owen

Plasma acetone turnover rates were measured with the primed continuous infusion of 2-[14C]acetone in patients with moderate to severe diabetic ketoacidosis. Plasma acetone turnover rates ranged from 1.52 to 15.9 mumol X kg-1 X min-1 (108-1038 mumol X 1.73 m-2 X min-1) and were directly related to the plasma acetone concentrations that ranged from 0.47 to 7.61 mM. The average acetone turnover rate was 6.45 mumol X kg-1 X min-1 (533 mumol X 1.73 m-2 X min-1), a value twice that obtained in a similar group of diabetic ketoacidotic patients via the single-injection technique of 2-[14C]acetone administration. Degradation of urine glucose revealed that 14C from administered 2-[14C )acetone was principally located in carbons 1, 2, 5, and 6 of the glucose molecule in five of six patients. This distribution is similar to that expected from 2-[14C]pyruvate, suggesting that acetone was converted to glucose through pyruvate. In one patient, label was located predominantly in glucose carbons 3 and 4, indicating that acetone metabolism may be different in some patients. Acetol (1-hydroxyacetone) and 1,2-propanediol (PPD), two possible metabolites of acetone, were detected in plasma of the patients. The concentrations of Acetol ranged from 0 to 0.48 mM and of PPD ranged from 0 to 0.53 mM. The concentrations of each metabolite were directly related to the plasma acetone concentrations. During the continuous infusion of 2-[14C]acetone, the specific activities of plasma glucose and PPD rose continuously but did not reach constant values. Estimates of the minimal percent plasma glucose and PPD derived from plasma acetone averaged 2.1 and 74%, respectively.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. M. Biemel, D. A. Friedl, and M. O. Lederer Identification and Quantification of Major Maillard Cross-links in Human Serum Albumin and Lens Protein. EVIDENCE FOR GLUCOSEPANE AS THE DOMINANT COMPOUND J. Biol. Chem., July 5, 2002; 277(28): 24907 - 24915. [Abstract] [Full Text] [PDF]

				K. C. Chang, K. S. Paek, H. J. Kim, Y. S. Lee, C. Yabe-Nishimura, and H. G. Seo Substrate-Induced Up-Regulation of Aldose Reductase by Methylglyoxal, a Reactive Oxoaldehyde Elevated in Diabetes Mol. Pharmacol., May 1, 2002; 61(5): 1184 - 1191. [Abstract] [Full Text] [PDF]

				P. A. Voziyan, T. O. Metz, J. W. Baynes, and B. G. Hudson A Post-Amadori Inhibitor Pyridoxamine Also Inhibits Chemical Modification of Proteins by Scavenging Carbonyl Intermediates of Carbohydrate and Lipid Degradation J. Biol. Chem., January 25, 2002; 277(5): 3397 - 3403. [Abstract] [Full Text] [PDF]

				N. Murata-Kamiya and H. Kamiya Methylglyoxal, an endogenous aldehyde, crosslinks DNA polymerase and the substrate DNA Nucleic Acids Res., August 15, 2001; 29(16): 3433 - 3438. [Abstract] [Full Text] [PDF]

				A. E. Kitabchi, G. E. Umpierrez, M. B. Murphy, E. J. Barrett, R. A. Kreisberg, J. I. Malone, and B. M. Wall Management of Hyperglycemic Crises in Patients With Diabetes Diabetes Care, January 1, 2001; 24(1): 131 - 153. [Full Text]

				T. Oya, N. Hattori, Y. Mizuno, S. Miyata, S. Maeda, T. Osawa, and K. Uchida Methylglyoxal Modification of Protein. CHEMICAL AND IMMUNOCHEMICAL CHARACTERIZATION OF METHYLGLYOXAL-ARGININE ADDUCTS J. Biol. Chem., June 25, 1999; 274(26): 18492 - 18502. [Abstract] [Full Text] [PDF]

				E. B. Frye, T. P. Degenhardt, S. R. Thorpe, and J. W. Baynes Role of the Maillard Reaction in Aging of Tissue Proteins. ADVANCED GLYCATION END PRODUCT-DEPENDENT INCREASE IN IMIDAZOLIUM CROSS-LINKS IN HUMAN LENS PROTEINS J. Biol. Chem., July 24, 1998; 273(30): 18714 - 18719. [Abstract] [Full Text] [PDF]

				W. Che, M. Asahi, M. Takahashi, H. Kaneto, A. Okado, S. Higashiyama, and N. Taniguchi Selective Induction of Heparin-binding Epidermal Growth Factor-like Growth Factor by Methylglyoxal and 3-Deoxyglucosone in Rat Aortic Smooth Muscle Cells. THE INVOLVEMENT OF REACTIVE OXYGEN SPECIES FORMATION AND A POSSIBLE IMPLICATION FOR ATHEROGENESIS IN DIABETES J. Biol. Chem., July 18, 1997; 272(29): 18453 - 18459. [Abstract] [Full Text] [PDF]