Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Levy, J.
Right arrow Articles by Avioli, L. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Levy, J.
Right arrow Articles by Avioli, L. V.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes, Vol 35, Issue 8 899-905, Copyright © 1986 by American Diabetes Association

ARTICLES

Ca2+-Mg2+-ATPase activity in kidney basolateral membrane in non-insulin-dependent diabetic rats. Effect of insulin

J Levy, JR Gavin, MR Hammerman and LV Avioli

The direct effect of insulin on the high-affinity Ca2+-Mg2+-ATPase was studied in kidney proximal tubular basolateral membranes (BLM) obtained from control and streptozocin-induced non-insulin-dependent diabetes mellitus (NIDDM) rats. Plasma glucose of the diabetic animals was only mildly elevated (217 +/- 9 vs. 138 +/- 3 mg/dl). Both high- and low-affinity calcium-dependent Ca2+-Mg2+-ATPase activities were identified in the BLM. Enzyme activity in BLM from diabetic rats was higher at all Ca2+ concentrations tested due to a higher maximum velocity of the enzyme from NIDDM rats. The high-affinity Ca2+-Mg2+-ATPase activity was inhibited by trifluoroperazine (TFP) in both membranes. No difference in calmodulin content was found in the membranes from the diabetic and control rats. Insulin (16-200 microU/ml) significantly increased the high-affinity Ca2+-Mg2+-ATPase activity (17-40%) in membranes from control animals but had no effect on the enzyme activity in the membranes from the NIDDM rats. The basal activity of the enzyme at 0.1 microM free Ca2+ was higher in the BLM from the NIDDM animals compared to controls (17.8 +/- 0.5 vs. 14.7 +/- 0.8 nM Pi X mg-1 X min-1; P less than .02). There was no effect of insulin on the Ca2+-independent ATPase activity of BLM preparations. These findings demonstrate a defect in the ability of insulin to regulate the high-affinity Ca2+-Mg2+-ATPase activity in BLM from diabetic rats. Such a defect in enzyme activity may play a role in the mechanism of impaired insulin action observed in these NIDDM rats.
Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Toxicol PatholHome page
S. Senthil, M. Sridevi, and K. V. Pugalendi
Cardioprotective Effect of Oleanolic Acid on Isoproterenol-Induced Myocardial Ischemia in Rats
Toxicol Pathol, April 1, 2007; 35(3): 418 - 423.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. Ishida, T. Ishida, N. Ono, H. Matsuura, M. Watanabe, G. Kajiyama, M. Kambe, and T. Oshima
Effects of Insulin on Calcium Metabolism and Platelet Aggregation
Hypertension, August 1, 1996; 28(2): 209 - 212.
[Abstract] [Full Text]

Home page
 HypertensionHome page
B. Grunfeld, M. Gimenez, M. Romo, L. Rabinovich, and R. B. Simsolo
Calcium-ATPase and Insulin in Adolescent Offspring of Essential Hypertensive Parents
Hypertension, December 1, 1995; 26(6): 1070 - 1073.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 1986 by the American Diabetes Association.