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Diabetes, Vol 37, Issue 5 563-568, Copyright © 1988 by American Diabetes Association

ARTICLES

Control of glucose phosphorylation and glucose usage in clonal insulinoma cells

T Shimizu, BB Knowles and FM Matschinsky
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104.

Glucose metabolism was investigated in two established clonal insulinoma cell lines (RINm5F and HIT) and in a newly developed line of mouse insulinoma cells (IgSV195). The hexokinase capacity in the homogenates of RINm5F cells was 22.1 +/- 3.23 U/g protein, but glucokinase was barely detectable (0.06 +/- 0.013 U/g protein). In contrast, both HIT and IgSV195 cells contained glucokinase (1.5 +/- 0.17 and 1.0 +/- 0.16 U/g protein, respectively) in addition to hexokinase activity. Glucose usage by the intact cells qualitatively reflected the glucose phosphorylation found in the cell-free extracts. RINm5F cells exhibited a high glucose usage rate with one high-affinity component, whereas both HIT and IgSV195 cells showed two components with different glucose affinities. HIT and IgSV195 cells may be useful for a model of pancreatic beta-cell glycolysis.
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This article has been cited by other articles:


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L. K. Olson, W. Schroeder, R. P. Robertson, N. D. Goldberg, and T. F. Walseth
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[Abstract] [Full Text] [PDF]


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Copyright © 1988 by the American Diabetes Association.