Diabetes, Vol 38, Issue 5 618-624, Copyright © 1989 by American Diabetes Association

ARTICLES

Failure of insulin infusion to stimulate fractional muscle protein synthesis in type I diabetic patients. Anabolic effect of insulin and decreased proteolysis

PJ Pacy, KS Nair, C Ford and D Halliday
Nutrition Research Group, Clinical Research Centre, Harrow, Middlesex, United Kingdom.

We evaluated the influence of insulin on fractional mixed skeletal muscle protein synthesis (FMPS) in eight type I (insulin-dependent) diabetic patients in the postabsorptive state. FMPS was calculated from the increment in [13C]leucine in mixed skeletal muscle protein obtained by serial percutaneous needle biopsy during a continuous 8-h intravenous infusion of L-[13C]leucine. We used the plasma [13C]-alpha-ketoisocaproate (representing intracellular leucine labeling) as the precursor pool of protein synthesis for our calculations. FMPS during the insulin treatment (0.0472 +/- 0.0046%/h; plasma glucose 4.6 +/- 1.0 mM) was not different from FMPS during insulin deprivation (0.0499 +/- 0.0046%/h; plasma glucose 16.4 +/- 0.5 mM). Using plasma [13C]-alpha-ketoisocaproate at isotopic plateau for calculation of leucine flux and as the precursor for leucine oxidation, we further confirmed the findings of our group and others that insulin treatment decreases leucine flux, leucine oxidation, and the nonoxidative portion of leucine flux. Our data on direct measurement of FMPS provide further evidence that the anabolic effect of insulin in the postabsorptive type I diabetic patient is mediated via reduction of proteolysis rather than by increasing protein synthesis.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z. Liu, G. Li, S. R. Kimball, L. A. Jahn, and E. J. Barrett Glucocorticoids modulate amino acid-induced translation initiation in human skeletal muscle Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E275 - E281. [Abstract] [Full Text] [PDF]

				W. Long, E. J. Barrett, L. Wei, and Z. Liu Adrenalectomy enhances the insulin sensitivity of muscle protein synthesis Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E102 - E109. [Abstract] [Full Text] [PDF]

				P. Halvatsiotis, K. R. Short, M. Bigelow, and K. S. Nair Synthesis Rate of Muscle Proteins, Muscle Functions, and Amino Acid Kinetics in Type 2 Diabetes Diabetes, August 1, 2002; 51(8): 2395 - 2404. [Abstract] [Full Text] [PDF]

				Y. Boirie, K. R. Short, B. Ahlman, M. Charlton, and K. S. Nair Tissue-Specific Regulation of Mitochondrial and Cytoplasmic Protein Synthesis Rates by Insulin Diabetes, December 1, 2001; 50(12): 2652 - 2658. [Abstract] [Full Text] [PDF]

				J. C. Kostyak, S. R. Kimball, L. S. Jefferson, and P. A. Farrell Severe diabetes inhibits resistance exercise-induced increase in eukaryotic initiation factor 2B activity J Appl Physiol, July 1, 2001; 91(1): 79 - 84. [Abstract] [Full Text] [PDF]

				T. Hillier, W. Long, L. Jahn, L. Wei, and E. J. Barrett Physiological Hyperinsulinemia Stimulates p70S6k Phosphorylation in Human Skeletal Muscle J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4900 - 4904. [Abstract] [Full Text]

				W. Long, L. Saffer, L. Wei, and E. J. Barrett Amino acids regulate skeletal muscle PHAS-I and p70 S6-kinase phosphorylation independently of insulin Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E301 - E306. [Abstract] [Full Text] [PDF]

				M. J. Fedele, J. M. Hernandez, C. H. Lang, T. C. Vary, S. R. Kimball, L. S. Jefferson, and P. A. Farrell Severe diabetes prohibits elevations in muscle protein synthesis after acute resistance exercise in rats J Appl Physiol, January 1, 2000; 88(1): 102 - 108. [Abstract] [Full Text] [PDF]

				P. A. Farrell, M. J. Fedele, J. Hernandez, J. D. Fluckey, J. L. Miller III, C. H. Lang, T. C. Vary, S. R. Kimball, and L. S. Jefferson Hypertrophy of skeletal muscle in diabetic rats in response to chronic resistance exercise J Appl Physiol, September 1, 1999; 87(3): 1075 - 1082. [Abstract] [Full Text] [PDF]

				D. W. Boyle, S. C. Denne, H. Moorehead, W.-H. Lee, R. R. Bowsher, and E. A. Liechty Effect of rhIGF-I infusion on whole fetal and fetal skeletal muscle protein metabolism in sheep Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E1082 - E1091. [Abstract] [Full Text] [PDF]

				M. Charlton and K. S. Nair Protein Metabolism in Insulin-Dependent Diabetes Mellitus J. Nutr., February 1, 1998; 128(2): 323 - 323. [Abstract] [Full Text]

				P. De Feo Fed State Protein Metabolism in Diabetes Mellitus J. Nutr., February 1, 1998; 128(2): 328 - 328. [Abstract] [Full Text]

				M. G. Vogiatzi, K. S. Nair, P. R. Beckett, and K. C. Copeland Insulin Does Not Stimulate Protein Synthesis Acutely in Prepubertal Children with Insulin-Dependent Diabetes Mellitus J. Clin. Endocrinol. Metab., December 1, 1997; 82(12): 4083 - 4087. [Abstract] [Full Text] [PDF]

				B. D. Roy, M. A. Tarnopolsky, J. D. Macdougall, J. Fowles, and K. E. Yarasheski Effect of glucose supplement timing on protein metabolism after resistance training J Appl Physiol, June 1, 1997; 82(6): 1882 - 1888. [Abstract] [Full Text] [PDF]

				A. Hyltander Invited Review: Intravenous Feeding Kinetics and Its Effects on Energy and Nitrogen Metabolism Nutr Clin Pract, April 1, 1994; 9(2): 51 - 57. [Abstract] [PDF]