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Diabetes, Vol 39, Issue 1 17-21, Copyright © 1990 by American Diabetes Association

ARTICLES

Immediate elimination of labile HbA1c with allosteric effectors of hemoglobin

K Nakashima, Y Hattori, K Yamazaki, M Takechi, Y Andoh and T Miyaji
Department of Laboratory Medicine, St. Luke's College of Nursing, Tokyo, Japan.

Our aim was to find a simple method of removing labile glycosylated hemoglobin (HbA1c) from blood samples before it is measured by cation-exchange chromatography. Labile HbA1c is formed by the binding of glucose to the NH2-terminal valine of the beta-chain of HbA. We sought a more competitive binder for the same site to dissociate labile HbA1c to glucose and HbA. Inorganic phosphates were found to have a strong allosteric effect and a great ability to eliminate labile HbA1c. We developed our method with 4 mM tetrapolyphosphate in the hemolyzing solution to eliminate labile HbA1c during the automatic processing (at pH 6 and heated for 2 min at 45 degrees C) of blood samples for HbA1c estimation. This may be useful when estimating HbA1c by the manual method.
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