Diabetes, Vol 39, Issue 1 87-95, Copyright © 1990 by American Diabetes Association

ARTICLES

Interaction between insulin and glucose-delivery route in regulation of net hepatic glucose uptake in conscious dogs

BA Adkins-Marshall, SR Myers, GK Hendrick, PE Williams, K Triebwasser, B Floyd and AD Cherrington
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0615.

In the presence of fixed basal levels of insulin, the route of intravenous glucose delivery (protal vs. peripheral) determines whether net hepatic glucose uptake (NHGU) occurs. Our aims were to determine if the route of intravenous glucose delivery also plays a role in regulating NHGU in the presence of hyperinsulinemia and to determine if length of fast (18 vs. 36 h) influences regulation of NHGU. Five conscious dogs fasted 18 h were given somatostatin and replacement insulin (245 +/- 34 microU.kg-1.min-1) and glucagon (0.65 ng.kg-1.min-1) infusions intraportally. After a 40-min control period, the insulin infusion rate was increased fourfold, and glucose was infused for 3 h. Glucose was given either through a peripheral vein or the portal vein for 90 min to double the glucose load reaching the liver. The order of infusions was randomized. NHGU was measured with the arterial - venous difference technique. Insulin and glucagon levels were 12 +/- 2, 35 +/- 6, and 36 +/- 5 microU/ml and 55 +/- 12, 61 +/- 13, and 59 +/- 7 pg/ml during the control, peripheral, and portal infusions, respectively. The glucose infusion rate, the load of glucose reaching the liver, and the arterial-portal plasma glucose gradient were 0, 9.58 +/- 2.28, and 10.44 +/- 2.94 mg.kg-1.min-1; 29.4 +/- 3.6, 56.8 +/- 3.4, and 56.8 +/- 2.8 mg.kg-1.min-1; and 2 +/- 1, 5 +/- 1, and -51 +/- 15 mg/dl during the same periods.(ABSTRACT TRUNCATED AT 250 WORDS)
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. A. DiCostanzo, D. P. Dardevet, D. W. Neal, M. Lautz, E. Allen, W. Snead, and A. D. Cherrington Role of the hepatic sympathetic nerves in the regulation of net hepatic glucose uptake and the mediation of the portal glucose signal Am J Physiol Endocrinol Metab, January 1, 2006; 290(1): E9 - E16. [Abstract] [Full Text] [PDF]

				J. M. Grubert, M. Lautz, D. B. Lacy, M. C. Moore, B. Farmer, A. Penaloza, A. D. Cherrington, and O. P. McGuinness Impact of continuous and pulsatile insulin delivery on net hepatic glucose uptake Am J Physiol Endocrinol Metab, August 1, 2005; 289(2): E232 - E240. [Abstract] [Full Text] [PDF]

				C. A. DiCostanzo, M. C. Moore, M. Lautz, M. Scott, B. Farmer, C. A. Everett, J. G. Still, A. Higgins, and A. D. Cherrington Simulated first-phase insulin release using Humulin or insulin analog HIM2 is associated with prolonged improvement in postprandial glycemia Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E46 - E52. [Abstract] [Full Text] [PDF]

				S.-S. Chen, C. J. Torres-Sanchez, N. Hosein, Y. Zhang, D. B. Lacy, and O. P. McGuinness Time course of the hepatic adaptation to TPN: interaction with glycogen depletion Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E163 - E170. [Abstract] [Full Text] [PDF]

				F. Zangeneh, R. Basu, P. Shah, P. Arora, M. Camilleri, and R. A. Rizza Enteral infusion of glucose at rates approximating EGP enhances glucose disposal but does not cause hypoglycemia Am J Physiol Endocrinol Metab, August 1, 2003; 285(2): E280 - E286. [Abstract] [Full Text] [PDF]

				T. K. T. Lam, G. Van de Werve, and A. Giacca Free fatty acids increase basal hepatic glucose production and induce hepatic insulin resistance at different sites Am J Physiol Endocrinol Metab, February 1, 2003; 284(2): E281 - E290. [Abstract] [Full Text] [PDF]

				N. Ogihara, S. Ebihara, W. Kawamura, M. Okamoto, T. Sakai, K. Takiguchi, T. Morita, R. Uchida, Y. Matsuyama, Y. Hayashi, et al. Characterization of the portal signal in a nonsteady hyperglycemic state in conscious dogs Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E148 - E155. [Abstract] [Full Text] [PDF]

				P. Georg, A. Kautzky-Willer, K. Mahdjoobian, A. Hofer, R. Prager, G. Pacini, and B. H. Ludvik Influence of Metabolic Control on Splanchnic Glucose Uptake, Insulin Sensitivity, and the Time Required for Glucose Absorption in Patients With Type 1 Diabetes Diabetes Care, November 1, 2002; 25(11): 2042 - 2047. [Abstract] [Full Text] [PDF]

				S. H. Kreisman, A. Manzon, S. J. Nessim, J. A. Morais, R. Gougeon, S. J. Fisher, M. Vranic, and E. B. Marliss Glucoregulatory responses to intense exercise performed in the postprandial state Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E786 - E793. [Abstract] [Full Text] [PDF]

				M. Shiota, P. Jackson, P. Galassetti, M. Scott, D. W. Neal, and A. D. Cherrington Combined intraportal infusion of acetylcholine and adrenergic blockers augments net hepatic glucose uptake Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E544 - E552. [Abstract] [Full Text] [PDF]

				P. Galassetti, Y. Koyama, R. H. Coker, D. B. Lacy, A. D. Cherrington, and D. H. Wasserman Role of a negative arterial-portal venous glucose gradient in the postexercise state Am J Physiol Endocrinol Metab, December 1, 1999; 277(6): E1038 - E1045. [Abstract] [Full Text] [PDF]

				S. Cardin, M. Emshwiller, P. A. Jackson, W. L. Snead, J. Hastings, D. S. Edgerton, and A. D. Cherrington Portal glucose infusion increases hepatic glycogen deposition in conscious unrestrained rats J Appl Physiol, October 1, 1999; 87(4): 1470 - 1475. [Abstract] [Full Text] [PDF]

				A. Manzon, S. J. Fisher, J. A. Morais, L. Lipscombe, M.-C. Guimond, S. J. Nessim, R. J. Sigal, J. B. Halter, M. Vranic, and E. B. Marliss Glucose infusion partially attenuates glucose production and increases uptake during intense exercise J Appl Physiol, August 1, 1998; 85(2): 511 - 524. [Abstract] [Full Text] [PDF]

				P. Galassetti, M. Shiota, B. A. Zinker, D. H. Wasserman, and A. D. Cherrington A negative arterial-portal venous glucose gradient decreases skeletal muscle glucose uptake Am J Physiol Endocrinol Metab, July 1, 1998; 275(1): E101 - E111. [Abstract] [Full Text] [PDF]

				R. Burcelin, C. Mrejen, J. F. Decaux, S. H. De Mouzon, J. Girard, and M. J. Charron In Vivo and in Vitro Regulation of Hepatic Glucagon Receptor mRNA Concentration by Glucose Metabolism J. Biol. Chem., April 3, 1998; 273(14): 8088 - 8093. [Abstract] [Full Text] [PDF]