Diabetes, Vol 39, Issue 6 697-701, Copyright © 1990 by American Diabetes Association

ARTICLES

Dependence of antigen expression on functional state of beta-cells

K Aaen, J Rygaard, K Josefsen, H Petersen, CH Brogren, T Horn and K Buschard
Bartholin Institute, Kommunehospitalet, Copenhagen K, Denmark.

Antigen expression corresponding to anti-islet cell surface monoclonal antibodies IC2 and A2B5 was studied. IC2 is a rat-rat hybridoma autoantibody produced from the BB rat; among islet cells, IC2 is beta-cell specific. A2B5 is an anti-ganglioside antibody described as labeling beta-cells. Islets of Langerhans from Lewis rats were isolated and cultured for 18 h in RPMI-1640 with five different glucose concentrations (2.2, 3.3, 5.5, 11.1, and 18.3 mM). In some experiments, islets were precultured for 2 or 3 days. After isolation of islet cells and antibody labeling, the percent of IC2+ beta-cells in the different groups increased from 33.3, 34.5, 40.9, and 57.2 to 58.6% (P less than 10(-6). For A2B5, the percent of labeled islet cells increased from 37.4, 41.8, 46.7, and 53.8 to 56.2% (P less than 10(-4). Thus, increasing glucose concentration leading to higher beta-cell activity implies an increase in antigen expression. Neither A2B5 nor IC2 reacts with insulin, as shown by absorption experiments and immune electron microscopy of binding sites. Electron microscopy of IC2-gold-labeled islet cells substantiated the beta-cell specificity of IC2. In conclusion, expression of the corresponding antigens to IC2 and A2B5 depends on the functional state of the beta-cells; because this has been shown to be an important factor in the development of insulin-dependent diabetes, our findings may be of potential pathogenetic interest.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. N. Samli, M. J. McGuire, C. B. Newgard, S. A. Johnston, and K. C. Brown Peptide-Mediated Targeting of the Islets of Langerhans Diabetes, July 1, 2005; 54(7): 2103 - 2108. [Abstract] [Full Text] [PDF]

				Diabetes Prevention Trial-Type 1 Diabetes Study Gr Effects of Insulin in Relatives of Patients with Type 1 Diabetes Mellitus N. Engl. J. Med., May 30, 2002; 346(22): 1685 - 1691. [Abstract] [Full Text] [PDF]

				A. Moore, S. Bonner-Weir, and R. Weissleder Noninvasive In Vivo Measurement of {beta}-Cell Mass in Mouse Model of Diabetes Diabetes, October 1, 2001; 50(10): 2231 - 2236. [Abstract] [Full Text]

				A. Amrani, S. Durant, M. Throsby, J. Coulaud, M. Dardenne, and F. Homo-Delarche Glucose Homeostasis in the Nonobese Diabetic Mouse at the Prediabetic Stage Endocrinology, March 1, 1998; 139(3): 1115 - 1124. [Abstract] [Full Text] [PDF]