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Diabetes, Vol 40, Issue 4 462-464, Copyright © 1991 by American Diabetes Association

ARTICLES

Vanadate induction of L-type pyruvate kinase mRNA in adult rat hepatocytes in primary culture

M Miralpeix, JF Decaux, A Kahn and R Bartrons
Department of Human Physiological Sciences and Nutrition, University of Barcelona, Spain.

In primary culture of adult rat hepatocytes, vanadate in the presence of glucose stimulates the expression of the liver (L-type) pyruvate kinase gene. Glucose by itself was inactive, and vanadate, like insulin, was also inefficient in the absence of glucose. Similar results were obtained on glucokinase gene expression. An analogue of cAMP, 8-(4-chlorophenylthio)-cAMP, inhibited the production of L-type pyruvate kinase and glucokinase mRNAs in the presence of glucose plus vanadate.
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Copyright © 1991 by the American Diabetes Association.