Diabetes, Vol 40, Issue 5 648-651, Copyright © 1991 by American Diabetes Association

ARTICLES

Exclusive expression of MHC class II proteins on CD45+ cells in pancreatic islets of NOD mice

MF McInerney, S Rath and CA Janeway
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510.

The expression of MHC class II molecules on beta-cells of the pancreatic islet has been proposed to play a role in the genesis of insulin-dependent diabetes mellitus in the NOD mouse. We investigated this by immunofluorescent double labeling of islet cells with anti-MHC and anti-CD45 to identify cells of hematopoietic origin. MHC class I expression increased with age on CD45- islet cells. MHC class II expression was not observed on CD45- islet cells at any age; the only cells in the islet that were MHC class II positive were also CD45+. This indicates that all MHC class II-positive cells in the islet are lymphoid cells that infiltrate the islet, whereas the islet endocrine cells express no MHC class II molecules. However, an increase in MHC class I expression occurred on beta-cells, and this may play a role in immunopathogenesis.
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