Diabetes, Vol 43, Issue 2 197-203, Copyright © 1994 by American Diabetes Association

ARTICLES

Islet-specific T-cell clones from the NOD mouse respond to beta-granule antigen

B Bergman and K Haskins
Barbara Davis Center for Childhood Diabetes, Denver, Colorado 80262.

Islet-reactive T-cell clones from NOD mice provide an important approach to the investigation of antigens with relevance to type I diabetes. To identify a source of beta-cell antigen suitable for biochemical studies, we have used two islet-specific, diabetogenic T-cell clones to test beta-tumor cells. beta-tumor cell lines, maintained in continuous culture, were found to lose antigenicity rapidly. However, cells harvested directly from beta-tumors arising spontaneously in the transgenic NOD/Lt-Tg(RIPTag)1Lt mouse proved to be a potent source of beta-cell antigen for the T-cell clones. Subcellular fractionation of beta-tumor cells showed that the T-cell antigen was highly enriched in the beta-granule fraction and that this activity was associated with the granule membrane.
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