Diabetes, Vol 43, Issue 2 305-312, Copyright © 1994 by American Diabetes Association

ARTICLES

An upstream element from the human insulin receptor gene promoter contains binding sites for C/EBP beta and NF-1

NJ Webster, Y Kong, KE Cameron and JL Resnik
Department of Medicine, University of California, San Diego, La Jolla 92093-0673.

We have shown previously that a 500-bp region of the human insulin receptor promoter (-0.3 to -1.8 kb) was able to stimulate transcription from a heterologous thymidine kinase promoter in HepG2 hepatoma cells but not in HeLa fibroblasts. Footprint analysis localized the transcription factor binding sites to a 36-bp region at -1420. In this paper, we analyze the factors that recognize this element and show that it contains binding sites for the CAAT/enhancer binding protein C/EBP and nuclear factor 1 (NF-1). In addition we show that both C/EBP alpha and the C/EBP beta can transactivate the human insulin receptor promoter in a dose-dependent manner.
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