Diabetes, Vol 44, Issue 8 878-883, Copyright © 1995 by American Diabetes Association

ARTICLES

Involvement of ATP-sensitive K+ channels in free radical-mediated inhibition of insulin secretion in rat pancreatic beta-cells

M Nakazaki, M Kakei, N Koriyama and H Tanaka
First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.

To explore the mechanisms of inhibition of insulin secretion in pancreatic beta-cells by oxygen free radicals, we studied the effects of H2O2 on membrane currents using the patch-clamp technique. Exposure of beta-cells to H2O2 (> or = 30 mumol/l) increased the activity of ATP-sensitive potassium (K+ATP) channels without changing the single channel conductance in cell-attached membrane patches. Action currents observed during superfusion of 11.1 mmol/l glucose were suppressed. In inside-out membrane patches, the activity of K+ATP channels was not influenced by H2O2. In conventional whole-cell clamp experiments using a pipette solution containing 3 mmol/l ATP, H2O2 did not influence the membrane currents. However, H2O2 did activate the K+ATP channel current in perforated whole-cell clamp configurations. The increased K+ATP channel current was reversed by subsequent exposure to 11.1 mmol/l 2-ketoisocaproic acid. In cell-attached membrane patches, the K+ATP channel current evoked by exposure to 30 mumol/l H2O2 was inhibited by exposure to 11.1 mmol/l glyceraldehyde, whereas the channel was again activated by exposure to 0.3 mmol/l H2O2. Subsequent superfusion of 11.1 mmol/l 2-ketoisocaproic acid inhibited the channel; this effect was counteracted by exposure to 10 mmol/l H2O2. Transient inhibition of K+ATP channels with provocation of action potentials was observed after washout of 100 mumol/l H2O2 during superfusion of 2.8 or 11.1 mmol/l glucose.(ABSTRACT TRUNCATED AT 250 WORDS)
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