Diabetes, Vol 47, Issue 1 98-103, Copyright © 1998 by American Diabetes Association

ARTICLES

Depot-specific differences in adipose tissue gene expression in lean and obese subjects

AM Lefebvre, M Laville, N Vega, JP Riou, L van Gaal, J Auwerx and H Vidal
INSERM U.325, Departement d'Atherosclerose, Institut Pasteur, Lille, France.

Intra-abdominal and subcutaneous adipose tissue display important metabolic differences that underlie the association of visceral, but not subcutaneous, fat with obesity-related cardiovascular and metabolic problems. Because the molecular mechanisms contributing to these differences are not yet defined, we compared by reverse transcription-polymerase chain reaction the expression of 15 mRNAs that encode proteins of known importance in adipocyte function in paired omental and subcutaneous abdominal biopsies. No difference in mRNA expression between omental and subcutaneous adipose tissue was observed for hormone sensitive lipase, lipoprotein lipase, 6-phosphofructo-1-kinase, insulin receptor substrate 1, p85alpha regulatory subunit of phosphatidylinositol-3-kinase, and Rad. Total amount of insulin receptor expression was significantly higher in omental adipose tissue. Most of this increase was accounted for by expression of the differentially spliced insulin receptor lacking exon 11, which is considered to transmit the insulin signal less efficiently than the insulin receptor with exon 11. Perhaps consistent with a less efficient insulin signaling, a twofold reduction in GLUT4, glycogen synthase, and leptin mRNA expression was observed in omental adipose tissue. Finally peroxisome proliferator activated receptor-gamma (PPAR-gamma) mRNA levels were significantly lower in visceral adipose tissue in subjects with a BMI <30 kg/m2, but not in obese subjects, indicating that relative PPAR-gamma expression is increased in omental fat in obesity. This suggests that altered expression of PPAR-gamma might play a role in adipose tissue distribution and expansion.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. A. Phillips, T. P. Ciaraldi, Deborah. K. Oh, M. K. Savu, and R. R. Henry Adiponectin secretion and response to pioglitazone is depot dependent in cultured human adipose tissue Am J Physiol Endocrinol Metab, October 1, 2008; 295(4): E842 - E850. [Abstract] [Full Text] [PDF]

				J. Staszkiewicz, J. M. Gimble, J. A. Manuel, and B. Gawronska-Kozak IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential Stem Cells, October 1, 2008; 26(10): 2666 - 2673. [Abstract] [Full Text] [PDF]

				R. MacLaren, W. Cui, S. Simard, and K. Cianflone Influence of obesity and insulin sensitivity on insulin signaling genes in human omental and subcutaneous adipose tissue J. Lipid Res., February 1, 2008; 49(2): 308 - 323. [Abstract] [Full Text] [PDF]

				J. Y. Kim, Y. Wu, and C. M. Smas Characterization of ScAP-23, a new cell line from murine subcutaneous adipose tissue, identifies genes for the molecular definition of preadipocytes Physiol Genomics, October 19, 2007; 31(2): 328 - 342. [Abstract] [Full Text] [PDF]

				W. Jia, H. Wu, Y. Bao, C. Wang, J. Lu, J. Zhu, and K. Xiang Association of Serum Retinol-Binding Protein 4 and Visceral Adiposity in Chinese Subjects with and without Type 2 Diabetes J. Clin. Endocrinol. Metab., August 1, 2007; 92(8): 3224 - 3229. [Abstract] [Full Text] [PDF]

				M. Laplante, W. T. Festuccia, G. Soucy, Y. Gelinas, J. Lalonde, J. P. Berger, and Y. Deshaies Mechanisms of the Depot Specificity of Peroxisome Proliferator-Activated Receptor {gamma} Action on Adipose Tissue Metabolism. Diabetes, October 1, 2006; 55(10): 2771 - 2778. [Abstract] [Full Text] [PDF]

				I. Mertens, R. V Considine, M. Van der Planken, and L. F Van Gaal Hemostasis and fibrinolysis in non-diabetic overweight and obese men and women. Is there still a role for leptin? Eur. J. Endocrinol., September 1, 2006; 155(3): 477 - 484. [Abstract] [Full Text] [PDF]

				S. Gesta, M. Bluher, Y. Yamamoto, A. W. Norris, J. Berndt, S. Kralisch, J. Boucher, C. Lewis, and C. R. Kahn Evidence for a role of developmental genes in the origin of obesity and body fat distribution PNAS, April 25, 2006; 103(17): 6676 - 6681. [Abstract] [Full Text] [PDF]

				F. H. Einstein, G. Atzmon, X.-m. Yang, X.-H. Ma, M. Rincon, E. Rudin, R. Muzumdar, and N. Barzilai Differential Responses of Visceral and Subcutaneous Fat Depots to Nutrients Diabetes, March 1, 2005; 54(3): 672 - 678. [Abstract] [Full Text] [PDF]

				Y. Miyazaki, E. De Filippis, M. Bajaj, E. Wajcberg, L. Glass, C. Triplitt, E. Cersosimo, L. J Mandarino, and R. A Defronzo Predictors of improved glycaemic control with rosiglitazone therapy in type 2 diabetic patients: a practical approach for the primary care physician The British Journal of Diabetes & Vascular Disease, January 1, 2005; 5(1): 28 - 35. [Abstract] [PDF]

				M. Grohmann, M. Sabin, J. Holly, J. Shield, E. Crowne, and C. Stewart Characterization of differentiated subcutaneous and visceral adipose tissue from children: the influences of TNF-{alpha} and IGF-I J. Lipid Res., January 1, 2005; 46(1): 93 - 103. [Abstract] [Full Text] [PDF]

				T. Tchkonia, Y. D. Tchoukalova, N. Giorgadze, T. Pirtskhalava, I. Karagiannides, R. A. Forse, A. Koo, M. Stevenson, D. Chinnappan, A. Cartwright, et al. Abundance of two human preadipocyte subtypes with distinct capacities for replication, adipogenesis, and apoptosis varies among fat depots Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E267 - E277. [Abstract] [Full Text] [PDF]

				L. Hutley, W. Shurety, F. Newell, R. McGeary, N. Pelton, J. Grant, A. Herington, D. Cameron, J. Whitehead, and J. Prins Fibroblast Growth Factor 1: A Key Regulator of Human Adipogenesis Diabetes, December 1, 2004; 53(12): 3097 - 3106. [Abstract] [Full Text] [PDF]

				B. J. Nicklas, B. W. J. H. Penninx, M. Cesari, S. B. Kritchevsky, A. B. Newman, A. M. Kanaya, M. Pahor, D. Jingzhong, and T. B. Harris Association of Visceral Adipose Tissue with Incident Myocardial Infarction in Older Men and Women: The Health, Aging and Body Composition Study Am. J. Epidemiol., October 15, 2004; 160(8): 741 - 749. [Abstract] [Full Text] [PDF]

				V. Giusti, M. Suter, C. Verdumo, R. C. Gaillard, P. Burckhardt, and F. P. Pralong Molecular Determinants of Human Adipose Tissue: Differences between Visceral and Subcutaneous Compartments in Obese Women J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1379 - 1384. [Abstract] [Full Text] [PDF]

				P. J. Larsen, P. B. Jensen, R. V. Sorensen, L. K. Larsen, N. Vrang, E. M. Wulff, and K. Wassermann Differential Influences of Peroxisome Proliferator-Activated Receptors{gamma} and -{alpha} on Food Intake and Energy Homeostasis Diabetes, September 1, 2003; 52(9): 2249 - 2259. [Abstract] [Full Text] [PDF]

				T.-B. Nguyen-Duy, M. Z. Nichaman, T. S. Church, S. N. Blair, and R. Ross Visceral fat and liver fat are independent predictors of metabolic risk factors in men Am J Physiol Endocrinol Metab, June 1, 2003; 284(6): E1065 - E1071. [Abstract] [Full Text] [PDF]

				M. Kolehmainen, M. I. J. Uusitupa, E. Alhava, M. Laakso, and H. Vidal Effect of the Pro12Ala Polymorphism in the Peroxisome Proliferator-Activated Receptor (PPAR) {gamma}2 Gene on the Expression of PPAR{gamma} Target Genes in Adipose Tissue of Massively Obese Subjects J. Clin. Endocrinol. Metab., April 1, 2003; 88(4): 1717 - 1722. [Abstract] [Full Text] [PDF]

				K. A. Virtanen, K. Hallsten, R. Parkkola, T. Janatuinen, F. Lonnqvist, T. Viljanen, T. Ronnemaa, J. Knuuti, R. Huupponen, P. Lonnroth, et al. Differential Effects of Rosiglitazone and Metformin on Adipose Tissue Distribution and Glucose Uptake in Type 2 Diabetic Subjects Diabetes, February 1, 2003; 52(2): 283 - 290. [Abstract] [Full Text] [PDF]

				M. L. Martelli, R. Iuliano, I. Le Pera, I. Sama', C. Monaco, S. Cammarota, T. Kroll, L. Chiariotti, M. Santoro, and A. Fusco Inhibitory Effects of Peroxisome Proliferator-Activated Receptor {gamma} on Thyroid Carcinoma Cell Growth J. Clin. Endocrinol. Metab., October 1, 2002; 87(10): 4728 - 4735. [Abstract] [Full Text] [PDF]

				M. Stumvoll and H. Haring The Peroxisome Proliferator-Activated Receptor-{gamma}2 Pro12Ala Polymorphism Diabetes, August 1, 2002; 51(8): 2341 - 2347. [Abstract] [Full Text] [PDF]

				Y. Miyazaki, A. Mahankali, M. Matsuda, S. Mahankali, J. Hardies, K. Cusi, L. J. Mandarino, and R. A. DeFronzo Effect of Pioglitazone on Abdominal Fat Distribution and Insulin Sensitivity in Type 2 Diabetic Patients J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2784 - 2791. [Abstract] [Full Text] [PDF]

				C. J. Lelliott, L. Logie, C. P. Sewter, D. Berger, P. Jani, F. Blows, S. O'Rahilly, and A. Vidal-Puig Lamin Expression in Human Adipose Cells in Relation to Anatomical Site and Differentiation State J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 728 - 734. [Abstract] [Full Text] [PDF]

				F. Diraison, E. Dusserre, H. Vidal, M. Sothier, and M. Beylot Increased hepatic lipogenesis but decreased expression of lipogenic gene in adipose tissue in human obesity Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E46 - E51. [Abstract] [Full Text] [PDF]

				C. J. de Souza, M. Eckhardt, K. Gagen, M. Dong, W. Chen, D. Laurent, and B. F. Burkey Effects of Pioglitazone on Adipose Tissue Remodeling Within the Setting of Obesity and Insulin Resistance Diabetes, August 1, 2001; 50(8): 1863 - 1871. [Abstract] [Full Text] [PDF]

				M. Caron, M. Auclair, C. Vigouroux, M. Glorian, C. Forest, and J. Capeau The HIV Protease Inhibitor Indinavir Impairs Sterol Regulatory Element-Binding Protein-1 Intranuclear Localization, Inhibits Preadipocyte Differentiation, and Induces Insulin Resistance Diabetes, June 1, 2001; 50(6): 1378 - 1388. [Abstract] [Full Text]

				P. Imbeault, H. Vidal, A. Tremblay, N. Vega, A. Nadeau, J.-P. Després, and P. Mauriège Age-Related Differences in Messenger Ribonucleic Acid Expression of Key Proteins Involved in Adipose Cell Differentiation and Metabolism J. Clin. Endocrinol. Metab., February 1, 2001; 86(2): 828 - 833. [Abstract] [Full Text]

				F. Caserta, T. Tchkonia, V. N. Civelek, M. Prentki, N. F. Brown, J. D. McGarry, R. A. Forse, B. E. Corkey, J. A. Hamilton, and J. L. Kirkland Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E238 - E247. [Abstract] [Full Text] [PDF]

				B. L. Wajchenberg Subcutaneous and Visceral Adipose Tissue: Their Relation to the Metabolic Syndrome Endocr. Rev., December 1, 2000; 21(6): 697 - 738. [Abstract] [Full Text]

				N. M. Lapsys, A. D. Kriketos, M. Lim-Fraser, A. M. Poynten, A. Lowy, S. M. Furler, D. J. Chisholm, and G. J. Cooney Expression of Genes Involved in Lipid Metabolism Correlate with Peroxisome Proliferator-Activated Receptor {gamma} Expression in Human Skeletal Muscle J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4293 - 4297. [Abstract] [Full Text]

				M. C. Henson and V. D. Castracane Leptin in Pregnancy Biol Reprod, November 1, 2000; 63(5): 1219 - 1228. [Abstract] [Full Text]

				M. Stumvoll, S. Jacob, H. G. Wahl, B. Hauer, K. Löblein, P. Grauer, R. Becker, M. Nielsen, W. Renn, and H. Häring Suppression of Systemic, Intramuscular, and Subcutaneous Adipose Tissue Lipolysis by Insulin in Humans J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3740 - 3745. [Abstract] [Full Text]

				L. B. Williams, R. L. Fawcett, A. S. Waechter, P. Zhang, B. E. Kogon, R. Jones, M. Inman, J. Huse, and R. V. Considine Leptin Production in Adipocytes from Morbidly Obese Subjects: Stimulation by Dexamethasone, Inhibition with Troglitazone, and Influence of Gender J. Clin. Endocrinol. Metab., August 1, 2000; 85(8): 2678 - 2684. [Abstract] [Full Text]

				E. D. Rosen, C. J. Walkey, P. Puigserver, and B. M. Spiegelman Transcriptional regulation of adipogenesis Genes & Dev., June 1, 2000; 14(11): 1293 - 1307. [Full Text]

				M. Nawano, A. Oku, K. Ueta, I. Umebayashi, T. Ishirahara, K. Arakawa, A. Saito, M. Anai, M. Kikuchi, and T. Asano Hyperglycemia contributes insulin resistance in hepatic and adipose tissue but not skeletal muscle of ZDF rats Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E535 - E543. [Abstract] [Full Text] [PDF]

				F. Krempler, D. Breban, H. Oberkofler, H. Esterbauer, E. Hell, B. Paulweber, and W. Patsch Leptin, Peroxisome Proliferator-Activated Receptor-{gamma}, and CCAAT/Enhancer Binding Protein-{alpha} mRNA Expression in Adipose Tissue of Humans and Their Relation to Cardiovascular Risk Factors Arterioscler. Thromb. Vasc. Biol., February 1, 2000; 20(2): 443 - 449. [Abstract] [Full Text] [PDF]

				A. M. Isidori, M. Caprio, F. Strollo, C. Moretti, G. Frajese, A. Isidori, and A. Fabbri Leptin and Androgens in Male Obesity: Evidence for Leptin Contribution to Reduced Androgen Levels J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3673 - 3680. [Abstract] [Full Text] [PDF]

				R. A. DeFronzo Pharmacologic Therapy for Type 2 Diabetes Mellitus Ann Intern Med, August 17, 1999; 131(4): 281 - 303. [Abstract] [Full Text] [PDF]

				H. H. Zhang, S. Kumar, A. H. Barnett, and M. C. Eggo Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake J. Clin. Endocrinol. Metab., July 1, 1999; 84(7): 2550 - 2556. [Abstract] [Full Text]

				Y. Wang, W. Lee-Kwon, J. L. Martindale, L. Adams, P. Heller, J. M. Egan, and M. Bernier Modulation of CCAAT/Enhancer-Binding Protein-{alpha} Gene Expression by Metabolic Signals in Rodent Adipocytes Endocrinology, July 1, 1999; 140(7): 2938 - 2947. [Abstract] [Full Text]

				S. Tartare-Deckert, C. Chavey, M.-N. Monthouel, N. Gautier, and E. Van Obberghen The Matricellular Protein SPARC/Osteonectin as a Newly Identified Factor Up-regulated in Obesity J. Biol. Chem., June 15, 2001; 276(25): 22231 - 22237. [Abstract] [Full Text] [PDF]