Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arden, S. D.
Right arrow Articles by Hutton, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arden, S. D.
Right arrow Articles by Hutton, J. C.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes, Vol 48, Issue 3 531-542, Copyright © 1999 by American Diabetes Association

ARTICLES

Molecular cloning of a pancreatic islet-specific glucose-6-phosphatase catalytic subunit-related protein

SD Arden, T Zahn, S Steegers, S Webb, B Bergman, RM O'Brien and JC Hutton
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.

A pancreatic islet-specific glucose-6-phosphatase-related protein (IGRP) was cloned using a subtractive cDNA expression cloning procedure from mouse insulinoma tissue. Two alternatively spliced variants that differed by the presence or absence of a 118-bp exon (exon IV) were detected in normal balb/c mice, diabetic ob/ob mice, and insulinoma tissue. The longer, 1901-bp full-length cDNA encoded a 355-amino acid protein (molecular weight 40,684) structurally related (50% overall identity) to the liver glucose-6-phosphatase and exhibited similar predicted transmembrane topology, conservation of catalytically important residues, and the presence of an endoplasmic reticulum retention signal. The shorter transcript encoded two possible open reading frames (ORFs), neither of which possessed His174, a residue thought to be the phosphoryl acceptor (Pan CJ, Lei KJ, Annabi B, Hemrika W, Chou JY: Transmembrane topology of glucose-6-phosphatase. J Biol Chem 273:6144-6148, 1998). Northern blot and reverse transcription-polymerase chain reaction analysis showed that the mRNA was highly expressed in pancreatic islets and expressed more in beta-cell lines than in an alpha-cell line. It was notably absent in tissues and cell lines of non-islet neuroendocrine origin, and no other major tissue source of the mRNA was found. During development, it was expressed in parallel with insulin mRNA. The mRNA was efficiently translated and glycosylated in an in vitro translation/membrane translocation system and readily transcribed into COS 1, HIT, and CHO cells using cytomegalovirus or Rous sarcoma virus promoters. Whereas the liver glucose-6-phosphatase showed activity in these transfection systems, the IGRP failed to show glucose phosphotransferase or phosphatase activity with p-nitrophenol phosphate, inorganic pyrophosphate, or a range of sugar phosphates hydrolyzed by the liver enzyme. While the metabolic function of the enzyme is not resolved, its remarkable tissue-specific expression warrants further investigation, as does its transcriptional regulation in conditions where glucose responsiveness of the pancreatic islet is altered.
Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DiabetesHome page
Y. Wang, B. P. Flemming, C. C. Martin, S. R. Allen, J. Walters, J. K. Oeser, J. C. Hutton, and R. M. O'Brien
Long-Range Enhancers Are Required to Maintain Expression of the Autoantigen Islet-Specific Glucose-6-Phosphatase Catalytic Subunit Related Protein in Adult Mouse Islets In Vivo
Diabetes, January 1, 2008; 57(1): 133 - 141.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Wenzlau, K. Juhl, L. Yu, O. Moua, S. A. Sarkar, P. Gottlieb, M. Rewers, G. S. Eisenbarth, J. Jensen, H. W. Davidson, et al.
The cation efflux transporter ZnT8 (Slc30A8) is a major autoantigen in human type 1 diabetes
PNAS, October 23, 2007; 104(43): 17040 - 17045.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wang, J. K. Oeser, C. Yang, S. Sarkar, S. I. Hackl, A. H. Hasty, O. P. McGuinness, W. Paradee, J. C. Hutton, D. R. Powell, et al.
Deletion of the Gene Encoding the Ubiquitously Expressed Glucose-6-phosphatase Catalytic Subunit-related Protein (UGRP)/Glucose-6-phosphatase Catalytic Subunit-beta Results in Lowered Plasma Cholesterol and Elevated Glucagon
J. Biol. Chem., December 29, 2006; 281(52): 39982 - 39989.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Yang, N. A. Danke, D. Berger, S. Reichstetter, H. Reijonen, C. Greenbaum, C. Pihoker, E. A. James, and W. W. Kwok
Islet-Specific Glucose-6-Phosphatase Catalytic Subunit-Related Protein-Reactive CD4+ T Cells in Human Subjects.
J. Immunol., March 1, 2006; 176(5): 2781 - 2789.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
B. T. Vander Kooi, H. Onuma, J. K. Oeser, C. A. Svitek, S. R. Allen, C. W. Vander Kooi, W. J. Chazin, and R. M. O'Brien
The Glucose-6-Phosphatase Catalytic Subunit Gene Promoter Contains Both Positive and Negative Glucocorticoid Response Elements
Mol. Endocrinol., December 1, 2005; 19(12): 3001 - 3022.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Mukherjee, D. Wagar, T. A. Stephens, E. Lee-Chan, and B. Singh
Identification of CD4+ T Cell-Specific Epitopes of Islet-Specific Glucose-6-Phosphatase Catalytic Subunit-Related Protein: A Novel {beta} Cell Autoantigen in Type 1 Diabetes
J. Immunol., May 1, 2005; 174(9): 5306 - 5315.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. C. Martin, J. K. Oeser, and R. M. O'Brien
Differential Regulation of Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein Gene Transcription by Pax-6 and Pdx-1
J. Biol. Chem., August 13, 2004; 279(33): 34277 - 34289.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. Frigeri, C. C. Martin, C. A. Svitek, J. K. Oeser, J. C. Hutton, M. Gannon, and R. M. O'Brien
The Proximal Islet-Specific Glucose-6-Phosphatase Catalytic Subunit-Related Protein Autoantigen Promoter Is Sufficient to Initiate but not Maintain Transgene Expression in Mouse Islets in Vivo
Diabetes, July 1, 2004; 53(7): 1754 - 1764.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Petrolonis, Q. Yang, P. J. Tummino, S. M. Fish, A. E. Prack, S. Jain, T. F. Parsons, P. Li, N. A. Dales, L. Ge, et al.
Enzymatic Characterization of the Pancreatic Islet-specific Glucose-6-Phosphatase-related Protein (IGRP)
J. Biol. Chem., April 2, 2004; 279(14): 13976 - 13983.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Ghosh, J.-J. Shieh, C.-J. Pan, and J. Y. Chou
Histidine 167 Is the Phosphate Acceptor in Glucose-6-phosphatase-{beta} Forming a Phosphohistidine Enzyme Intermediate during Catalysis
J. Biol. Chem., March 26, 2004; 279(13): 12479 - 12483.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Figge, F. Lammert, B. Paigen, A. Henkel, S. Matern, R. Korstanje, B. L. Shneider, F. Chen, E. Stoltenberg, K. Spatz, et al.
Hepatic Overexpression of Murine Abcb11 Increases Hepatobiliary Lipid Secretion and Reduces Hepatic Steatosis
J. Biol. Chem., January 23, 2004; 279(4): 2790 - 2799.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. C. Hutton and G. S. Eisenbarth
A pancreatic {beta}-cell-specific homolog of glucose-6-phosphatase emerges as a major target of cell-mediated autoimmunity in diabetes
PNAS, July 22, 2003; 100(15): 8626 - 8628.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. M. Lieberman, A. M. Evans, B. Han, T. Takaki, Y. Vinnitskaya, J. A. Caldwell, D. V. Serreze, J. Shabanowitz, D. F. Hunt, S. G. Nathenson, et al.
Identification of the {beta} cell antigen targeted by a prevalent population of pathogenic CD8+ T cells in autoimmune diabetes
PNAS, July 8, 2003; 100(14): 8384 - 8388.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. T. Vander Kooi, R. S. Streeper, C. A. Svitek, J. K. Oeser, D. R. Powell, and R. M. O'Brien
The Three Insulin Response Sequences in the Glucose-6-phosphatase Catalytic Subunit Gene Promoter Are Functionally Distinct
J. Biol. Chem., March 28, 2003; 278(14): 11782 - 11793.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. Shiroi, M. Yoshikawa, H. Yokota, H. Fukui, S. Ishizaka, K. Tatsumi, and Y. Takahashi
Identification of Insulin-Producing Cells Derived from Embryonic Stem Cells by Zinc-Chelating Dithizone
Stem Cells, July 1, 2002; 20(4): 284 - 292.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. A. Hornbuckle, D. S. Edgerton, J. E. Ayala, C. A. Svitek, J. K. Oeser, D. W. Neal, S. Cardin, A. D. Cherrington, and R. M. O'Brien
Selective tonic inhibition of G-6-Pase catalytic subunit, but not G-6-P transporter, gene expression by insulin in vivo
Am J Physiol Endocrinol Metab, October 1, 2001; 281(4): E713 - E725.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. J. Bischof, C. C. Martin, C. A. Svitek, B. T. Stadelmaier, L. A. Hornbuckle, J. K. Goldman, J. K. Oeser, J. C. Hutton, and R. M. O’Brien
Characterization of the Mouse Islet-Specific Glucose-6-Phosphatase Catalytic Subunit-Related Protein Gene Promoter by In Situ Footprinting: Correlation With Fusion Gene Expression in the Islet-Derived {beta}TC-3 and Hamster Insulinoma Tumor Cell Lines
Diabetes, March 1, 2001; 50(3): 502 - 514.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
C. C. Martin, L. J. Bischof, B. Bergman, L. A. Hornbuckle, C. Hilliker, C. Frigeri, D. Wahl, C. A. Svitek, R. Wong, J. K. Goldman, et al.
Cloning and Characterization of the Human and Rat Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein (IGRP) Genes
J. Biol. Chem., June 29, 2001; 276(27): 25197 - 25207.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 1999 by the American Diabetes Association.