Diabetes, Vol 48, Issue 4 714-721, Copyright © 1999 by American Diabetes Association

ARTICLES

Glucose entrainment of high-frequency plasma insulin oscillations in control and type 2 diabetic subjects

CS Mao, N Berman, K Roberts and E Ipp
Department of Medicine, Harbor-UCLA Medical Center, Torrance, California 90509-2910, USA.

Regular high-frequency oscillations of insulin secretion are characteristic of normal beta-cell function. These oscillations are easily entrainable to an exogenous rhythm by small changes in glucose concentration in vitro. We tested whether high-frequency insulin oscillations in vivo would also be entrainable by glucose and whether a lack of entrainment would characterize the diabetic beta-cell. We tested 13 control subjects and 11 patients with type 2 diabetes. Subjects underwent serial blood sampling at 1-min intervals for 60-120 min in the basal state or with small (15 mg/kg) boluses of glucose injected intravenously at exact 29-min intervals. Time series analysis was carried out using spectral analysis. Oscillations of basal plasma glucose concentrations were observed in both control and type 2 diabetic subjects, with a mean period of 11.3 +/- 3.1 and 11.6 +/- 2.0 min, respectively. These oscillations were entrained to mean periods of 15.0 +/- 0.6 and 14.2 +/- 0.9 min, respectively, by exogenous glucose. Regular high-frequency insulin oscillations were observed in control subjects; the mean period of basal plasma insulin oscillations was 10.7 +/- 1.2 min and was entrained to exogenously injected glucose, with a period of 15.2 +/- 0.1 min. In contrast, in the type 2 diabetic subjects, spontaneous insulin oscillations were unchanged by the glucose rhythm; the mean periods were 10.0 +/- 1.0 min during the basal period, and 10.1 +/- 0.0 min during glucose injections. These results demonstrate that spontaneous high-frequency insulin oscillations can be successfully entrained by glucose in control subjects. However, these oscillations in type 2 diabetic subjects are not similarly entrained. We conclude that loss of entrainment of spontaneous high-frequency insulin oscillations in type 2 diabetes is a highly sensitive manifestation of beta-cell secretory dysfunction.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. A. Ritzel, J. D. Veldhuis, and P. C. Butler The mass, but not the frequency, of insulin secretory bursts in isolated human islets is entrained by oscillatory glucose exposure Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E750 - E756. [Abstract] [Full Text] [PDF]

				V. Bancila, T. Cens, D. Monnier, F. Chanson, C. Faure, Y. Dunant, and A. Bloc Two SUR1-specific Histidine Residues Mandatory for Zinc-induced Activation of the Rat KATP Channel J. Biol. Chem., March 11, 2005; 280(10): 8793 - 8799. [Abstract] [Full Text] [PDF]

				C. S. Mao, N. Berman, and E. Ipp Loss of entrainment of high-frequency plasma insulin oscillations in type 2 diabetes is likely a glucose-specific {beta}-cell defect Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E50 - E54. [Abstract] [Full Text] [PDF]

				C. B. Juhl, M. Hollingdal, J. Sturis, G. Jakobsen, H. Agerso, J. Veldhuis, N. Porksen, and O. Schmitz Bedtime Administration of NN2211, a Long-Acting GLP-1 Derivative, Substantially Reduces Fasting and Postprandial Glycemia in Type 2 Diabetes Diabetes, February 1, 2002; 51(2): 424 - 429. [Abstract] [Full Text] [PDF]

				P. Gilon, M. A. Ravier, J.-C. Jonas, and J.-C. Henquin Control Mechanisms of the Oscillations of Insulin Secretion In Vitro and In Vivo Diabetes, February 1, 2002; 51(90001): S144 - 151. [Abstract] [Full Text] [PDF]

				N. Porksen, M. Hollingdal, C. Juhl, P. Butler, J. D. Veldhuis, and O. Schmitz Pulsatile Insulin Secretion: Detection, Regulation, and Role in Diabetes Diabetes, February 1, 2002; 51(90001): S245 - 254. [Abstract] [Full Text] [PDF]

				C. Juhl, T. Grofte, P. C. Butler, J. D. Veldhuis, O. Schmitz, and N. Porksen Effects of Fasting on Physiologically Pulsatile Insulin Release in Healthy Humans Diabetes, February 1, 2002; 51(90001): S255 - 257. [Abstract] [Full Text] [PDF]

				C. B. Juhl, N. Porksen, S. M. Pincus, A. P. Hansen, J. D. Veldhuis, and O. Schmitz Acute and Short-Term Administration of a Sulfonylurea (Gliclazide) Increases Pulsatile Insulin Secretion in Type 2 Diabetes Diabetes, August 1, 2001; 50(8): 1778 - 1784. [Abstract] [Full Text] [PDF]

				N. Porksen, C. Juhl, M. Hollingdal, S. M. Pincus, J. Sturis, J. D. Veldhuis, and O. Schmitz Concordant induction of rapid in vivo pulsatile insulin secretion by recurrent punctuated glucose infusions Am J Physiol Endocrinol Metab, January 1, 2000; 278(1): E162 - E170. [Abstract] [Full Text] [PDF]