Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miles, P. D.G.
Right arrow Articles by Olefsky, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miles, P. D.G.
Right arrow Articles by Olefsky, J. M.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Diabetes 50:139-142, 2001
© 2001 by the American Diabetes Association, Inc.

Exercise-Stimulated Glucose Turnover in the Rat Is Impaired by Glucosamine Infusion

Philip D.G. Miles, Katsuya Higo, and Jerrold M. Olefsky

From the Departments of Surgery (P.D.G.M.) and Medicine (K.H., J.M.O.), University of California-San Diego; the Division of Endocrinology and Metabolism (J.M.O.), San Diego VA Medical Center, San Diego; and the Whittier Diabetes Institute (J.M.O.), La Jolla, California.

Address correspondence and reprint requests to Dr. Philip D.G. Miles, Department of Surgery, UCSD Medical Center, 200 West Arbor Dr., San Diego, CA 92103-8400. E-mail: pmiles{at}ucsd.edu .

The infusion of glucosamine causes insulin resistance, presumably by entering the hexosamine biosynthetic pathway; it has been proposed that this pathway plays a role in hyperglycemia-induced insulin resistance. This study was undertaken to determine if glucosamine infusion could influence exercise-stimulated glucose uptake. Male SD rats were infused with glucosamine at 0.1 mg · kg-1 · min-1 (low-GlcN group), 6.5 mg · kg-1 · min-1 (high-GlcN group), or saline (control group) for 6.5 h and exercised on a treadmill for 30 min (17 m/min) at the end of the infusion period. Glucosamine infusion caused a modest increase in basal glycemia in both experimental groups, with no change in tracer-determined basal glucose turnover. During exercise, glucose turnover increased ~2.2-fold from 46 ± 2 to 101 ± 5 µmol · kg-1 · min-1 in the control group. Glucose turnover increased to a lesser extent in the glucosamine groups and was limited to 88% of control in the low-GlcN group (47 ± 2 to 90 ± 3 µmol · kg-1 · min-1; P < 0.01) and 72% of control in the high-GlcN group (43 ± 1 to 73 ± 3 µmol · kg-1 · min-1; P < 0.01). Similarly, the metabolic clearance rate (MCR) in the control group increased 72% from 6.1 ± 0.2 to 10.5 ± 0.7 ml · kg-1 · min-1 in response to exercise. However, the increase in MCR was only 83% of control in the low-GlcN group (5.2 ± 0.5 to 8.7 ± 0.5 ml · kg-1 · min-1; P < 0.01) and 59% of control in the high-GlcN group (4.5 ± 0.2 to 6.2 ± 0.3 ml · kg-1 · min-1; P < 0.01). Neither glucosamine infusion nor exercise significantly affected plasma insulin or free fatty acid (FFA) concentrations. In conclusion, the infusion of glucosamine, which is known to cause insulin resistance, also impaired exercise-induced glucose uptake. This inhibition was independent of hyperglycemia and FFA levels.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2001 by the American Diabetes Association.