Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Erratum (v52,p2449)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wojtaszewski, J. F.P.
Right arrow Articles by Richter, E. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wojtaszewski, J. F.P.
Right arrow Articles by Richter, E. A.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes 50:265-269, 2001
© 2001 by the American Diabetes Association, Inc.

Regulation of Glycogen Synthase Kinase-3 in Human Skeletal Muscle

Effects of Food Intake and Bicycle Exercise

Jørgen F.P. Wojtaszewski, Pernille Nielsen, Bente Kiens, and Erik A. Richter

From the Copenhagen Muscle Research Centre, Department of Human Physiology, University of Copenhagen, Copenhagen, Denmark.

Studies of skeletal muscle from rodents performed both in vivo and in vitro suggest a regulatory role of glycogen synthase kinase (GSK) 3 in glycogen synthase (GS) activation in response to insulin. Recently, hyper-insulinemic clamp studies in humans support such a role under nearly physiological conditions. In addition, in rats the activation of GS in skeletal muscle during treadmill running is time-related to the deactivation of GSK3. We investigated whether GSK3 was deactivated in human muscle during low- (~50% Vo2max for 1.5 h) and high-intensity (~75% Vo2max for 1 h) bicycle exercise as well as food intake. We observed a small but significant increase in GSK3{alpha} (10-20%) activity in biopsies obtained from vastus lateralis after both low- and high-intensity exercise, whereas GSK3ß activity was unaffected. Subsequent food intake increased Aktphosphorylation (~2-fold) and deactivated GSK3{alpha} (~40%), whereas GSK3ß activity was unchanged. GS activity increased in response to both exercise and food intake. We conclude that GSK3{alpha} but not GSK3ß may have a role in the regulation of GS activity in response to meal-associated hyperinsulinemia in humans. However, in contrast to findings in muscle from rats, exercise does not deactivate GSK3 in humans, suggesting a GSK3-independent mechanism in the regulation of GS activity in muscle during physical activity.

Address correspondence and reprint requests to Jørgen F.P. Wojtaszewski, Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, 13, Universitetsparken, DK-2100 Copenhagen, Denmark. Email: jwojtaszewski{at}aki.ku.dk .


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
T. P. Ciaraldi, S. E. Nikoulina, R. A. Bandukwala, L. Carter, and R. R. Henry
Role of Glycogen Synthase Kinase-3{alpha} in Insulin Action in Cultured Human Skeletal Muscle Cells
Endocrinology, September 1, 2007; 148(9): 4393 - 4399.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. T. Treebak, J. B. Birk, A. J. Rose, B. Kiens, E. A. Richter, and J. F. P. Wojtaszewski
AS160 phosphorylation is associated with activation of {alpha}2beta2{gamma}1- but not {alpha}2beta2{gamma}3-AMPK trimeric complex in skeletal muscle during exercise in humans
Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E715 - E722.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. P. Ciaraldi, D. K. Oh, L. Christiansen, S. E. Nikoulina, A. P. S. Kong, S. Baxi, S. Mudaliar, and R. R. Henry
Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E891 - E898.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Sakamoto, D. E. Arnolds, N. Fujii, H. F. Kramer, M. F. Hirshman, and L. J. Goodyear
Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E1031 - E1037.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Wilson, M. Hargreaves, and K. F. Howlett
Exercise does not alter subcellular localization, but increases phosphorylation of insulin-signaling proteins in human skeletal muscle
Am J Physiol Endocrinol Metab, February 1, 2006; 290(2): E341 - E346.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. E. Black, E. Mitchell, P. S. Freedson, S. R. Chipkin, and B. Braun
Improved insulin action following short-term exercise training: role of energy and carbohydrate balance
J Appl Physiol, December 1, 2005; 99(6): 2285 - 2293.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. Creer, P. Gallagher, D. Slivka, B. Jemiolo, W. Fink, and S. Trappe
Influence of muscle glycogen availability on ERK1/2 and Akt signaling after resistance exercise in human skeletal muscle
J Appl Physiol, September 1, 2005; 99(3): 950 - 956.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
A. J. Rose and E. A. Richter
Skeletal Muscle Glucose Uptake During Exercise: How is it Regulated?
Physiology, August 1, 2005; 20(4): 260 - 270.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. Y. Christ-Roberts, T. Pratipanawatr, W. Pratipanawatr, R. Berria, R. Belfort, and L. J. Mandarino
Increased insulin receptor signaling and glycogen synthase activity contribute to the synergistic effect of exercise on insulin action
J Appl Physiol, December 1, 2003; 95(6): 2519 - 2529.
[Abstract] [Full Text]

Home page
 Diabetes CareHome page
S. K. Gan, A. D. Kriketos, B. A. Ellis, C. H. Thompson, E. W. Kraegen, and D. J. Chisholm
Changes in Aerobic Capacity and Visceral Fat but not Myocyte Lipid Levels Predict Increased Insulin Action After Exercise in Overweight and Obese Men
Diabetes Care, June 1, 2003; 26(6): 1706 - 1713.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. Hojlund, P. Staehr, B. F. Hansen, K. A. Green, D. G. Hardie, E. A. Richter, H. Beck-Nielsen, and J. F.P. Wojtaszewski
Increased Phosphorylation of Skeletal Muscle Glycogen Synthase at NH2-Terminal Sites During Physiological Hyperinsulinemia in Type 2 Diabetes
Diabetes, June 1, 2003; 52(6): 1393 - 1402.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. Halse, L. G.D. Fryer, J. G. McCormack, D. Carling, and S. J. Yeaman
Regulation of Glycogen Synthase by Glucose and Glycogen: A Possible Role for AMP-Activated Protein Kinase
Diabetes, January 1, 2003; 52(1): 9 - 15.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-B. Kim, G. I. Shulman, and B. B. Kahn
Fatty Acid Infusion Selectively Impairs Insulin Action on Akt1 and Protein Kinase C lambda /zeta but Not on Glycogen Synthase Kinase-3
J. Biol. Chem., August 30, 2002; 277(36): 32915 - 32922.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Sakamoto and L. J. Goodyear
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Intracellular signaling in contracting skeletal muscle
J Appl Physiol, July 1, 2002; 93(1): 369 - 383.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. F. P. Wojtaszewski, J. N. Nielsen, and E. A. Richter
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Effect of acute exercise on insulin signaling and action in humans
J Appl Physiol, July 1, 2002; 93(1): 384 - 392.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. E. Hardt and J. Sadoshima
Glycogen Synthase Kinase-3{beta}: A Novel Regulator of Cardiac Hypertrophy and Development
Circ. Res., May 31, 2002; 90(10): 1055 - 1063.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2001 by the American Diabetes Association.