Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamagishi, S.-i.
Right arrow Articles by Brownlee, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamagishi, S.-i.
Right arrow Articles by Brownlee, M.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Diabetes 50:1491-1494, 2001
© 2001 by the American Diabetes Association, Inc.

Original Articles

Hyperglycemia Potentiates Collagen-Induced Platelet Activation Through Mitochondrial Superoxide Overproduction

Sho-ichi Yamagishi, Diane Edelstein, Xue-liang Du, and Michael Brownlee

Department of Medicine, Diabetes Research Center, Albert Einstein College of Medicine, Bronx, New York

Alteration of platelet function contributes to microthrombus formation and may play an important role in the pathogenesis of diabetic micro- and macroangiopathies. However, the molecular mechanism for platelet dysfunction observed in patients with diabetes has not been fully elucidated. In this study, the direct effects of hyperglycemia on platelet function in vitro were investigated. Hyperglycemia increased reactive oxygen species generation in human platelets, and this effect was additive with that of collagen. Thenoyltrifluoroacetone (TTFA), an inhibitor of mitochondrial electron transport chain complex II, and carbonyl cyanide m-chlorophenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, completely prevented the effects of hyperglycemia, suggesting that reactive oxygen species arise from the mitochondrial electron transport chain. Hyperglycemia potentiated both platelet aggregation and the subsequent release of platelet-derived growth factor AB induced by a nonaggregating subthreshold concentration of collagen, which were also completely inhibited by TTFA or CCCP. Furthermore, hyperglycemia was found to inhibit protein tyrosine phosphatase (PTP) activity and increase phosphorylation of the tyrosine kinase Syk in platelets exposed to collagen. Hyperglycemia-induced PTP inhibition and Syk phosphorylation were found to be completely prevented by TTFA, CCCP, or Mn(III)tetrakis (4-benzoic acid) porphyrin, a stable cell-permeable superoxide dismutase mimetic. These results suggest that hyperglycemia-induced mitochondrial superoxide generation may play an important role in platelet dysfunction observed in patients with diabetes.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DiabetesHome page
A. Benani, S. Troy, M. C. Carmona, X. Fioramonti, A. Lorsignol, C. Leloup, L. Casteilla, and L. Penicaud
Role for Mitochondrial Reactive Oxygen Species in Brain Lipid Sensing: Redox Regulation of Food Intake
Diabetes, January 1, 2007; 56(1): 152 - 160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Xia, H. Wang, H. J. Goldberg, S. Munk, I. G. Fantus, and C. I. Whiteside
Mesangial cell NADPH oxidase upregulation in high glucose is protein kinase C dependent and required for collagen IV expression
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F345 - F356.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Gorin, K. Block, J. Hernandez, B. Bhandari, B. Wagner, J. L. Barnes, and H. E. Abboud
Nox4 NAD(P)H Oxidase Mediates Hypertrophy and Fibronectin Expression in the Diabetic Kidney
J. Biol. Chem., November 25, 2005; 280(47): 39616 - 39626.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. Suleiman, D. Aronson, R. Asleh, M. R. Kapeliovich, A. Roguin, S. R. Meisel, M. Shochat, A. Sulieman, S. A. Reisner, W. Markiewicz, et al.
Haptoglobin Polymorphism Predicts 30-Day Mortality and Heart Failure in Patients With Diabetes and Acute Myocardial Infarction
Diabetes, September 1, 2005; 54(9): 2802 - 2806.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. W. Zmijewski, D. R. Moellering, C. L. Goffe, A. Landar, A. Ramachandran, and V. M. Darley-Usmar
Oxidized LDL induces mitochondrially associated reactive oxygen/nitrogen species formation in endothelial cells
Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H852 - H861.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. Cai, J. Wang, Y. Li, X. Sun, L. Wang, Z. Zhou, and Y. J. Kang
Inhibition of Superoxide Generation and Associated Nitrosative Damage Is Involved in Metallothionein Prevention of Diabetic Cardiomyopathy
Diabetes, June 1, 2005; 54(6): 1829 - 1837.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Jaramillo, M. Godbout, and M. Olivier
Hemozoin Induces Macrophage Chemokine Expression through Oxidative Stress-Dependent and -Independent Mechanisms
J. Immunol., January 1, 2005; 174(1): 475 - 484.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Hua, S. Munk, H. Goldberg, I. G. Fantus, and C. I. Whiteside
High Glucose-suppressed Endothelin-1 Ca2+ Signaling via NADPH Oxidase and Diacylglycerol-sensitive Protein Kinase C Isozymes in Mesangial Cells
J. Biol. Chem., September 5, 2003; 278(36): 33951 - 33962.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
J. E. Gerich
Clinical Significance, Pathogenesis, and Management of Postprandial Hyperglycemia
Arch Intern Med, June 9, 2003; 163(11): 1306 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Parthasarathi, H. Ichimura, S. Quadri, A. Issekutz, and J. Bhattacharya
Mitochondrial Reactive Oxygen Species Regulate Spatial Profile of Proinflammatory Responses in Lung Venular Capillaries
J. Immunol., December 15, 2002; 169(12): 7078 - 7086.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. Krotz, H. Y. Sohn, T. Gloe, S. Zahler, T. Riexinger, T. M. Schiele, B. F. Becker, K. Theisen, V. Klauss, and U. Pohl
NAD(P)H oxidase-dependent platelet superoxide anion release increases platelet recruitment
Blood, July 18, 2002; 100(3): 917 - 924.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. G. Rosca, V. M. Monnier, L. I. Szweda, and M. F. Weiss
Alterations in renal mitochondrial respiration in response to the reactive oxoaldehyde methylglyoxal
Am J Physiol Renal Physiol, July 1, 2002; 283(1): F52 - F59.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-i. Yamagishi, Y. Inagaki, T. Okamoto, S. Amano, K. Koga, M. Takeuchi, and Z. Makita
Advanced Glycation End Product-induced Apoptosis and Overexpression of Vascular Endothelial Growth Factor and Monocyte Chemoattractant Protein-1 in Human-cultured Mesangial Cells
J. Biol. Chem., May 31, 2002; 277(23): 20309 - 20315.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
H. D. White and C. K. Wong
Risk stratification and treatment benefits in patients with non-ST-elevation acute coronary syndromes
Eur. Heart J., February 1, 2002; 23(3): 187 - 191.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2001 by the American Diabetes Association.