Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aschenbach, W. G.
Right arrow Articles by Goodyear, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aschenbach, W. G.
Right arrow Articles by Goodyear, L. J.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Diabetes 51:567-573, 2002
© 2002 by the American Diabetes Association, Inc.

Effect of AICAR Treatment on Glycogen Metabolism in Skeletal Muscle

William G. Aschenbach, Michael F. Hirshman, Nobuharu Fujii, Kei Sakamoto, Kirsten F. Howlett, and Laurie J. Goodyear

From the Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts

AMP-activated protein kinase (AMPK) is proposed to stimulate fat and carbohydrate catabolism to maintain cellular energy status. Recent studies demonstrate that pharmacologic activation of AMPK and mutations in the enzyme are associated with elevated muscle glycogen content in vivo. Our purpose was to determine the mechanism for increased muscle glycogen associated with AMPK activity in vivo. AMPK activity and glycogen metabolism were studied in red and white gastrocnemius muscles from rats treated with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) in vivo, and also in muscles incubated with AICAR in vitro. In vivo AICAR treatment reduced blood glucose and increased blood lactate compared with basal values. AICAR increased muscle {alpha}2 AMPK activity, glycogen, and glucose-6-phosphate concentrations. Glycogen synthase activity was increased in the red gastrocnemius but was decreased in the white gastrocnemius. Glycogen phosphorylase activity increased in both muscles, with an inhibition initially observed in the red gastrocnemius. In vitro incubation with AICAR activated {alpha}2 AMPK but had no effect on either glycogen synthase or glycogen phosphorylase. These results suggest that AICAR treatment does not promote glycogen accumulation in skeletal muscle in vivo by altering glycogen synthase and glycogen phosphorylase. Rather, the increased glycogen is due to the well-known effects of AICAR to increase glucose uptake.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. S. Jaswal, M. Gandhi, B. A. Finegan, J. R. B. Dyck, and A. S. Clanachan
p38 mitogen-activated protein kinase mediates adenosine-induced alterations in myocardial glucose utilization via 5'-AMP-activated protein kinase
Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1978 - H1985.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Arad, C. E. Seidman, and J.G. Seidman
AMP-Activated Protein Kinase in the Heart: Role During Health and Disease
Circ. Res., March 2, 2007; 100(4): 474 - 488.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. Miyamoto, T. Toyoda, T. Hayashi, S. Yonemitsu, M. Nakano, S. Tanaka, K. Ebihara, H. Masuzaki, K. Hosoda, Y. Ogawa, et al.
Effect of acute activation of 5'-AMP-activated protein kinase on glycogen regulation in isolated rat skeletal muscle
J Appl Physiol, March 1, 2007; 102(3): 1007 - 1013.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Suwa, T. Egashira, H. Nakano, H. Sasaki, and S. Kumagai
Metformin increases the PGC-1{alpha} protein and oxidative enzyme activities possibly via AMPK phosphorylation in skeletal muscle in vivo
J Appl Physiol, December 1, 2006; 101(6): 1685 - 1692.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H.-J. Koh, D. E. Arnolds, N. Fujii, T. T. Tran, M. J. Rogers, N. Jessen, Y. Li, C. W. Liew, R. C. Ho, M. F. Hirshman, et al.
Skeletal Muscle-Selective Knockout of LKB1 Increases Insulin Sensitivity, Improves Glucose Homeostasis, and Decreases TRB3
Mol. Cell. Biol., November 15, 2006; 26(22): 8217 - 8227.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Fediuc, M. P. Gaidhu, and R. B. Ceddia
Inhibition of Insulin-Stimulated Glycogen Synthesis by 5-Aminoimidasole-4-Carboxamide-1-{beta}-D-Ribofuranoside-Induced Adenosine 5'-Monophosphate-Activated Protein Kinase Activation: Interactions with Akt, Glycogen Synthase Kinase 3-3{alpha}/{beta}, and Glycogen Synthase in Isolated Rat Soleus Muscle
Endocrinology, November 1, 2006; 147(11): 5170 - 5177.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. S. Jaswal, M. Gandhi, B. A. Finegan, J. R. B. Dyck, and A. S. Clanachan
Effects of adenosine on myocardial glucose and palmitate metabolism after transient ischemia: role of 5'-AMP-activated protein kinase
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1883 - H1892.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. R. Hancock, E. Janssen, and R. L. Terjung
Contraction-mediated phosphorylation of AMPK is lower in skeletal muscle of adenylate kinase-deficient mice
J Appl Physiol, February 1, 2006; 100(2): 406 - 413.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. C. Smith, C. R. Bruce, and D. J. Dyck
AMP kinase activation with AICAR simultaneously increases fatty acid and glucose oxidation in resting rat soleus muscle
J. Physiol., June 1, 2005; 565(2): 537 - 546.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. R. Pencek, J. Shearer, R. C. Camacho, F. D. James, D. B. Lacy, P. T. Fueger, E. P. Donahue, W. Snead, and D. H. Wasserman
5-Aminoimidazole-4-Carboxamide-1-{beta}-D-Ribofuranoside Causes Acute Hepatic Insulin Resistance In Vivo
Diabetes, February 1, 2005; 54(2): 355 - 360.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. B. Jorgensen, J. N. Nielsen, J. B. Birk, G. S. Olsen, B. Viollet, F. Andreelli, P. Schjerling, S. Vaulont, D. G. Hardie, B. F. Hansen, et al.
The {alpha}2-5'AMP-Activated Protein Kinase Is a Site 2 Glycogen Synthase Kinase in Skeletal Muscle and Is Responsive to Glucose Loading
Diabetes, December 1, 2004; 53(12): 3074 - 3081.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. R. Steinberg, A. C. Smith, B. J. W. van Denderen, Z. Chen, S. Murthy, D. J. Campbell, G. J. F. Heigenhauser, D. J. Dyck, and B. E. Kemp
AMP-Activated Protein Kinase Is Not Down-Regulated in Human Skeletal Muscle of Obese Females
J. Clin. Endocrinol. Metab., September 1, 2004; 89(9): 4575 - 4580.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. A. Iglesias, S. M. Furler, G. J. Cooney, E. W. Kraegen, and J.-M. Ye
AMP-Activated Protein Kinase Activation by AICAR Increases Both Muscle Fatty Acid and Glucose Uptake in White Muscle of Insulin-Resistant Rats In Vivo
Diabetes, July 1, 2004; 53(7): 1649 - 1654.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Hojlund, K. J. Mustard, P. Staehr, D. G. Hardie, H. Beck-Nielsen, E. A. Richter, and J. F. P. Wojtaszewski
AMPK activity and isoform protein expression are similar in muscle of obese subjects with and without type 2 diabetes
Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E239 - E244.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
M. Arad, I. P. Moskowitz, V. V. Patel, F. Ahmad, A. R. Perez-Atayde, D. B. Sawyer, M. Walter, G. H. Li, P. G. Burgon, C. T. Maguire, et al.
Transgenic Mice Overexpressing Mutant PRKAG2 Define the Cause of Wolff-Parkinson-White Syndrome in Glycogen Storage Cardiomyopathy
Circulation, June 10, 2003; 107(22): 2850 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. L. Longnus, R. B. Wambolt, H. L. Parsons, R. W. Brownsey, and M. F. Allard
5-Aminoimidazole-4-carboxamide 1-beta -D-ribofuranoside (AICAR) stimulates myocardial glycogenolysis by allosteric mechanisms
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2003; 284(4): R936 - R944.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. Halse, L. G.D. Fryer, J. G. McCormack, D. Carling, and S. J. Yeaman
Regulation of Glycogen Synthase by Glucose and Glycogen: A Possible Role for AMP-Activated Protein Kinase
Diabetes, January 1, 2003; 52(1): 9 - 15.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Stoppani, A. L. Hildebrandt, K. Sakamoto, D. Cameron-Smith, L. J. Goodyear, and P. D. Neufer
AMP-activated protein kinase activates transcription of the UCP3 and HKII genes in rat skeletal muscle
Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1239 - E1248.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Sakamoto and L. J. Goodyear
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Intracellular signaling in contracting skeletal muscle
J Appl Physiol, July 1, 2002; 93(1): 369 - 383.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2002 by the American Diabetes Association.