HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gao, G. Articles by Ma, J.-x. Search for Related Content PubMed PubMed Citation Articles by Gao, G. Articles by Ma, J.-x. Social Bookmarking                What's this?

Difference in Ischemic Regulation of Vascular Endothelial Growth Factor and Pigment Epithelium--Derived Factor in Brown Norway and Sprague Dawley Rats Contributing to Different Susceptibilities to Retinal Neovascularization

¹ Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina
² Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, Maryland

The present study compared susceptibilities of Sprague Dawley (SD) and Brown Norway (BN) rats with ischemia-induced retinal neovascularization. An exposure to constant hyperoxia followed by normoxia induced significant retinal neovascularization in BN rats but not in SD rats, as demonstrated by fluorescein retinal angiography, measurement of avascular area, and count of preretinal vascular cells. These results indicate a rat strain difference in susceptibility to retinal neovascularization. To understand the molecular basis responsible for the strain difference, we have measured the levels of pigment epithelium-derived factor (PEDF), an angiogenic inhibitor, and vascular endothelial growth factor (VEGF), a major angiogenic stimulator in the retina. The hyperoxia-treated BN rats showed a significant reduction in retinal PEDF, but they showed a substantial increase of VEGF at both the protein and RNA levels, resulting in an increased VEGF-to-PEDF ratio. Hyperoxia-treated SD rats showed changes in PEDF and VEGF levels that were less in magnitude and of shorter duration than in BN rats. In age-matched normal BN and SD rats, however, there was no detectable difference in the basal VEGF-to-PEDF ratio between the strains. These observations support the idea that different regulation of angiogenic inhibitors and stimulators under ischemia are responsible for the differences in susceptibility to ischemia-induced retinal neovascularization in SD and BN rats.

CiteULike

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				K. Lu, Y. Zhou, K. Kaufman, R. Mott, and J.-x. Ma Rat strain-dependent susceptibility to ischemia-induced retinopathy associated with retinal vascular endothelial growth factor regulation J. Mol. Endocrinol., April 1, 2007; 38(4): 423 - 432. [Abstract] [Full Text] [PDF]

				P. van Wijngaarden, H. M. Brereton, D. J. Coster, and K. A. Williams Genetic Influences on Susceptibility to Oxygen-Induced Retinopathy Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1761 - 1766. [Abstract] [Full Text] [PDF]

				S. X Zhang, J. J Wang, G. Gao, K. Parke, and J.-x. Ma Pigment epithelium-derived factor downregulates vascular endothelial growth factor (VEGF) expression and inhibits VEGF-VEGF receptor 2 binding in diabetic retinopathy. J. Mol. Endocrinol., August 1, 2006; 37(1): 1 - 12. [Abstract] [Full Text] [PDF]

				L. Notari, A. Miller, A. Martinez, J. Amaral, M. Ju, G. Robinson, L. E. H. Smith, and S. P. Becerra Pigment Epithelium-Derived Factor Is a Substrate for Matrix Metalloproteinase Type 2 and Type 9: Implications for Downregulation in Hypoxia Invest. Ophthalmol. Vis. Sci., August 1, 2005; 46(8): 2736 - 2747. [Abstract] [Full Text] [PDF]

				D. M. Lang, J. Blackledge, and R. W. Arnold Is Pacific Race a Retinopathy of Prematurity Risk Factor? Arch Pediatr Adolesc Med, August 1, 2005; 159(8): 771 - 773. [Abstract] [Full Text] [PDF]

				P. van Wijngaarden, D. J. Coster, H. M. Brereton, I. L. Gibbins, and K. A. Williams Strain-Dependent Differences in Oxygen-Induced Retinopathy in the Inbred Rat Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1445 - 1452. [Abstract] [Full Text] [PDF]

				S. X. Zhang, J.-x. Ma, J. Sima, Y. Chen, M. S. Hu, A. Ottlecz, and G. N. Lambrou Genetic Difference in Susceptibility to the Blood-Retina Barrier Breakdown in Diabetes and Oxygen-Induced Retinopathy Am. J. Pathol., January 1, 2005; 166(1): 313 - 321. [Abstract] [Full Text] [PDF]

				P. Baluk, C. G. Lee, H. Link, E. Ator, A. Haskell, J. A. Elias, and D. M. McDonald Regulated Angiogenesis and Vascular Regression in Mice Overexpressing Vascular Endothelial Growth Factor in Airways Am. J. Pathol., October 1, 2004; 165(4): 1071 - 1085. [Abstract] [Full Text] [PDF]

				H. Liu, J.-G. Ren, W. L. Cooper, C. E. Hawkins, M. R. Cowan, and P. Y. Tong Identification of the antivasopermeability effect of pigment epithelium-derived factor and its active site PNAS, April 27, 2004; 101(17): 6605 - 6610. [Abstract] [Full Text] [PDF]

				K. Ohno-Matsui, T. Uetama, T. Yoshida, M. Hayano, T. Itoh, I. Morita, and M. Mochizuki Reduced Retinal Angiogenesis in MMP-2-Deficient Mice Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5370 - 5375. [Abstract] [Full Text] [PDF]

				Y.-B. Shui, X. Wang, J. S. Hu, S.-P. Wang, C. M. Garcia, J. D. Potts, Y. Sharma, and D. C. Beebe Vascular Endothelial Growth Factor Expression and Signaling in the Lens Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 3911 - 3919. [Abstract] [Full Text] [PDF]