Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsumoto, M.
Right arrow Articles by Kasuga, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsumoto, M.
Right arrow Articles by Kasuga, M.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes 51:1672-1680, 2002
© 2002 by the American Diabetes Association, Inc.

Role of the Insulin Receptor Substrate 1 and Phosphatidylinositol 3-Kinase Signaling Pathway in Insulin-Induced Expression of Sterol Regulatory Element Binding Protein 1c and Glucokinase Genes in Rat Hepatocytes

Michihiro Matsumoto, Wataru Ogawa, Kiyoshi Teshigawara, Hiroshi Inoue, Kazuaki Miyake, Hiroshi Sakaue, and Masato Kasuga

From the Department of Clinical Molecular Medicine, Division of Diabetes, Digestive, and Kidney Diseases, Kobe University Graduate School of Medicine, Kobe, Japan

The mechanism by which insulin induces the expression of the sterol regulatory element binding protein 1c (SREBP-1c) and glucokinase genes was investigated in cultured rat hepatocytes. Overexpression of an NH2-terminal fragment of IRS-1 that contains the pleckstrin homology and phosphotyrosine binding domains (insulin receptor substrate-1 NH2-terminal fragment [IRS-1N]) inhibited insulin-induced tyrosine phosphorylation of IRS-1 as well as the association of IRS-1 with phosphatidylinositol (PI) 3-kinase activity, whereas the tyrosine phosphorylation of IRS-2 and its association with PI 3-kinase activity were slightly enhanced. The equivalent fragment of IRS-2 (IRS-2N) prevented insulin-induced tyrosine phosphorylation of both IRS-1 and IRS-2, although that of IRS-1 was inhibited more efficiently. The insulin-induced increases in the abundance of SREBP-1c and glucokinase mRNAs, both of which were sensitive to a dominant-negative mutant of PI 3-kinase, were blocked in cells in which the insulin-induced tyrosine phosphorylation of IRS-1 was inhibited by IRS-1N or IRS-2N. A dominant-negative mutant of Akt enhanced insulin-induced tyrosine phosphorylation of IRS-1 (but not that of IRS-2) and its association with PI 3-kinase activity, suggesting that Akt contributes to negative feedback regulation of IRS-1. The Akt mutant also promoted the effects of insulin on the accumulation of SREBP-1c and glucokinase mRNAs. These results suggest that the IRS-1–PI 3-kinase pathway is essential for insulin-induced expression of SREBP-1c and glucokinase genes.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DiabetesHome page
Y. Okamoto, W. Ogawa, A. Nishizawa, H. Inoue, K. Teshigawara, S. Kinoshita, Y. Matsuki, E. Watanabe, R. Hiramatsu, H. Sakaue, et al.
Restoration of Glucokinase Expression in the Liver Normalizes Postprandial Glucose Disposal in Mice With Hepatic Deficiency of PDK1
Diabetes, April 1, 2007; 56(4): 1000 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. Fukui, T. Wada, S. Kagawa, K. Nagira, M. Ikubo, H. Ishihara, M. Kobayashi, and T. Sasaoka
Impact of the Liver-Specific Expression of SHIP2 (SH2-Containing Inositol 5'-Phosphatase 2) on Insulin Signaling and Glucose Metabolism in Mice
Diabetes, July 1, 2005; 54(7): 1958 - 1967.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S.-S. Im, S.-Y. Kang, S.-Y. Kim, H.-i. Kim, J.-W. Kim, K.-S. Kim, and Y.-H. Ahn
Glucose-Stimulated Upregulation of GLUT2 Gene Is Mediated by Sterol Response Element-Binding Protein-1c in the Hepatocytes
Diabetes, June 1, 2005; 54(6): 1684 - 1691.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Inukai, Y. Nakashima, M. Watanabe, N. Takata, T. Sawa, S. Kurihara, T. Awata, and S. Katayama
Regulation of adiponectin receptor gene expression in diabetic mice
Am J Physiol Endocrinol Metab, May 1, 2005; 288(5): E876 - E882.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. H. Kim, M. J. Song, E. J. Yoo, S. S. Choe, S. D. Park, and J. B. Kim
Regulatory Role of Glycogen Synthase Kinase 3 for Transcriptional Activity of ADD1/SREBP1c
J. Biol. Chem., December 10, 2004; 279(50): 51999 - 52006.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Nadeau, J. W. Leitner, I. Gurerich, and B. Draznin
Insulin Regulation of Sterol Regulatory Element-binding Protein-1 Expression in L-6 Muscle Cells and 3T3 L1 Adipocytes
J. Biol. Chem., August 13, 2004; 279(33): 34380 - 34387.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. R. Commerford, L. Peng, J. J. Dube, and R. M. O'Doherty
In vivo regulation of SREBP-1c in skeletal muscle: effects of nutritional status, glucose, insulin, and leptin
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R218 - R227.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
I. Guillet-Deniau, A.-L. Pichard, A. Kone, C. Esnous, M. Nieruchalski, J. Girard, and C. Prip-Buus
Glucose induces de novo lipogenesis in rat muscle satellite cells through a sterol-regulatory-element-binding-protein-1c-dependent pathway
J. Cell Sci., April 15, 2004; 117(10): 1937 - 1944.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Sasaoka, T. Wada, K. Fukui, S. Murakami, H. Ishihara, R. Suzuki, K. Tobe, T. Kadowaki, and M. Kobayashi
SH2-containing Inositol Phosphatase 2 Predominantly Regulates Akt2, and Not Akt1, Phosphorylation at the Plasma Membrane in Response to Insulin in 3T3-L1 Adipocytes
J. Biol. Chem., April 9, 2004; 279(15): 14835 - 14843.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
T. Matsuzaka, H. Shimano, N. Yahagi, M. Amemiya-Kudo, H. Okazaki, Y. Tamura, Y. Iizuka, K. Ohashi, S. Tomita, M. Sekiya, et al.
Insulin-Independent Induction of Sterol Regulatory Element-Binding Protein-1c Expression in the Livers of Streptozotocin-Treated Mice
Diabetes, March 1, 2004; 53(3): 560 - 569.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Xu, V. Chang, S. B. Joseph, C. Trujillo, S. Bassilian, M. F. Saad, W. N. P. Lee, and I. J. Kurland
Peroxisomal Proliferator-Activated Receptor {alpha} Deficiency Diminishes Insulin-Responsiveness of Gluconeogenic/Glycolytic/Pentose Gene Expression and Substrate Cycle Flux
Endocrinology, March 1, 2004; 145(3): 1087 - 1095.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Sakaue, A. Nishizawa, W. Ogawa, K. Teshigawara, T. Mori, Y. Takashima, T. Noda, and M. Kasuga
Requirement for 3-Phosphoinositide-dependent Kinase-1 (PDK-1) in Insulin-induced Glucose Uptake in Immortalized Brown Adipocytes
J. Biol. Chem., October 3, 2003; 278(40): 38870 - 38874.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2002 by the American Diabetes Association.