|
 |
|
|||||||
|
|||||||||
Diabetes 51:2703-2708, 2002
© 2002 by the American Diabetes Association, Inc.
Isoform-Specific Regulation of 5' AMP-Activated Protein Kinase in Skeletal Muscle From Obese Zucker (fa/fa) Rats in Response to Contraction
1 Department of Clinical Physiology and Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
2 MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, U.K.
Glucose transport can be activated in skeletal muscle in response to insulin via activation of phosphoinositide (PI) 3-kinase and in response to contractions or hypoxia, presumably via activation of 5' AMP-activated protein kinase (AMPK). We determined the effects of insulin and muscle contraction/hypoxia on PI 3-kinase, AMPK, and glucose transport activity in epitrochlearis skeletal muscle from insulin-resistant Zucker (fa/ fa) rats. Insulin-stimulated glucose transport in isolated skeletal muscle was reduced 47% in obese versus lean rats, with a parallel 42% reduction in tyrosine-associated PI 3-kinase activity. Contraction and hypoxia elicited normal responses for glucose transport in skeletal muscle from insulin-resistant obese rats. Isoform-specific AMPK activity was measured in skeletal muscle in response to insulin, contraction, or hypoxia. Contraction increased AMPK
1 activity 2.3-fold in lean rats, whereas no effect was noted in obese rats. Hypoxia increased AMPK1 activity to a similar extent (more than sixfold) in lean and obese rats. Regardless of genotype, contraction, and hypoxia, each increased AMPK2 activity more than fivefold, whereas insulin did not alter either AMPK1 or -2 activity in skeletal muscle. In conclusion, obesity-related insulin resistance is associated with an isoform-specific impairment in AMPK1 in response to contraction. However, this impairment does not appear to affect contraction-stimulated glucose transport. Activation of AMPK2 in response to muscle contraction/ exercise is associated with a parallel and normal increase in glucose transport in insulin-resistant skeletal muscle.
CiteULike 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X.-X. Han, A. Chabowski, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Metabolic challenges reveal impaired fatty acid metabolism and translocation of FAT/CD36 but not FABPpm in obese Zucker rat muscle Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E566 - E575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sriwijitkamol, D. K. Coletta, E. Wajcberg, G. B. Balbontin, S. M. Reyna, J. Barrientes, P. A. Eagan, C. P. Jenkinson, E. Cersosimo, R. A. DeFronzo, et al. Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 Diabetes: A Time-Course and Dose-Response Study Diabetes, March 1, 2007; 56(3): 836 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Al-Khalili, K. Bouzakri, S. Glund, F. Lonnqvist, H. A. Koistinen, and A. Krook Signaling Specificity of Interleukin-6 Action on Glucose and Lipid Metabolism in Skeletal Muscle Mol. Endocrinol., December 1, 2006; 20(12): 3364 - 3375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Canto, A. V. Chibalin, B. R. Barnes, S. Glund, E. Suarez, J. W. Ryder, M. Palacin, J. R. Zierath, A. Zorzano, and A. Guma Neuregulins Mediate Calcium-induced Glucose Transport during Muscle Contraction J. Biol. Chem., August 4, 2006; 281(31): 21690 - 21697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sriwijitkamol, J. L. Ivy, C. Christ-Roberts, R. A. DeFronzo, L. J. Mandarino, and N. Musi LKB1-AMPK signaling in muscle from obese insulin-resistant Zucker rats and effects of training Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E925 - E932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Fisher, J.-S. Ju, P. J. Oppelt, J. L. Smith, A. Suzuki, and H. Esumi Muscle contractions, AICAR, and insulin cause phosphorylation of an AMPK-related kinase Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E986 - E992. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Ryder, Y. C. Long, E. Nilsson, M. Mahlapuu, and J. R. Zierath Effects of calcineurin activation on insulin-, AICAR- and contraction-induced glucose transport in skeletal muscle J. Physiol., September 1, 2005; 567(2): 379 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Barnes, S. Glund, Y. C. Long, G. Hjalm, L. Andersson, and J. R. Zierath 5'-AMP-activated protein kinase regulates skeletal muscle glycogen content and ergogenics FASEB J, May 1, 2005; 19(7): 773 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang and G. D. Holman Insulin and Contraction Stimulate Exocytosis, but Increased AMP-activated Protein Kinase Activity Resulting from Oxidative Metabolism Stress Slows Endocytosis of GLUT4 in Cardiomyocytes J. Biol. Chem., February 11, 2005; 280(6): 4070 - 4078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Leng, T. L. Steiler, and J. R. Zierath Effects of Insulin, Contraction, and Phorbol Esters on Mitogen-Activated Protein Kinase Signaling in Skeletal Muscle From Lean and ob/ob Mice Diabetes, June 1, 2004; 53(6): 1436 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yu, N. Fujii, M. F. Hirshman, J. M. Pomerleau, and L. J. Goodyear Cloning and characterization of mouse 5'-AMP-activated protein kinase {gamma}3 subunit Am J Physiol Cell Physiol, February 1, 2004; 286(2): C283 - C292. [Abstract] [Full Text]
|
|
|
|
|
|
H. A. Koistinen, D. Galuska, A. V. Chibalin, J. Yang, J. R. Zierath, G. D. Holman, and H. Wallberg-Henriksson 5-Amino-Imidazole Carboxamide Riboside Increases Glucose Transport and Cell-Surface GLUT4 Content in Skeletal Muscle From Subjects With Type 2 Diabetes Diabetes, May 1, 2003; 52(5): 1066 - 1072. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Diabetes | Diabetes Care | Clinical Diabetes | Diabetes Spectrum |