Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Konrad, D.
Right arrow Articles by Klip, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Konrad, D.
Right arrow Articles by Klip, A.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes 51:2719-2726, 2002
© 2002 by the American Diabetes Association, Inc.

Need for GLUT4 Activation to Reach Maximum Effect of Insulin-Mediated Glucose Uptake in Brown Adipocytes Isolated From GLUT4myc-Expressing Mice

Daniel Konrad1,2, Philip J. Bilan1, Zafar Nawaz1, Gary Sweeney1, Wenyan Niu1, Zhi Liu1, Costin N. Antonescu1, Assaf Rudich1, and Amira Klip1,3

1 Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada
2 Institute of Medical Sciences, University of Toronto, Toronto, Ontario, Canada
3 Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

There is a need to understand whether the amount of GLUT4 at the cell surface determines the extent of glucose uptake in response to insulin. Thus, we created a heterozygous mouse expressing modest levels of myc-tagged GLUT4 (GLUT4myc) in insulin-sensitive tissues under the control of the human GLUT4 promoter. Insulin stimulated 2-deoxyglucose uptake 6.5-fold in isolated brown adipocytes. GLUT1 did not contribute to the insulin response. The stimulation by insulin was completely blocked by wortmannin and partly (55 ± 2%) by the p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580. Insulin increased surface exposure of GLUT4myc twofold (determined by fluorescent or enzyme-linked myc immunodetection in intact adipocytes). Such increase was completely blocked by wortmannin but insensitive to SB203580. Insulin increased the kinase activity of the p38 MAPK ß-isoform 1.9-fold without affecting p38-{alpha}. In summary, the GLUT4myc mouse is a promising model for measuring GLUT4 translocation in intact primary cells. It affords direct comparison between GLUT4 translocation and glucose uptake in similar cell preparations, allowing one to study the regulation of GLUT4 activity. Using this animal model, we found that stimulation of glucose uptake into brown adipocytes involves both GLUT4 translocation and activation.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. S. Jaswal, M. Gandhi, B. A. Finegan, J. R. B. Dyck, and A. S. Clanachan
p38 mitogen-activated protein kinase mediates adenosine-induced alterations in myocardial glucose utilization via 5'-AMP-activated protein kinase
Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1978 - H1985.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Xiong, Q. F. Collins, J. An, E. Lupo Jr., H.-Y. Liu, D. Liu, J. Robidoux, Z. Liu, and W. Cao
p38 Mitogen-activated Protein Kinase Plays an Inhibitory Role in Hepatic Lipogenesis
J. Biol. Chem., February 16, 2007; 282(7): 4975 - 4982.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. J. Feldman, R. S. Streeper, R. V. Farese Jr., and K. R. Yamamoto
Myostatin modulates adipogenesis to generate adipocytes with favorable metabolic effects
PNAS, October 17, 2006; 103(42): 15675 - 15680.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L.-Z. Liu, H.-L. Zhao, J. Zuo, S. K.S. Ho, J. C.N. Chan, Y. Meng, F.-D. Fang, and P. C.Y. Tong
Protein Kinase C{zeta} Mediates Insulin-induced Glucose Transport through Actin Remodeling in L6 Muscle Cells
Mol. Biol. Cell, May 1, 2006; 17(5): 2322 - 2330.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. Turban, V. A. Beardmore, J. M. Carr, K. Sakamoto, E. Hajduch, J. S. C. Arthur, and H. S. Hundal
Insulin-Stimulated Glucose Uptake Does Not Require p38 Mitogen-Activated Protein Kinase in Adipose Tissue or Skeletal Muscle
Diabetes, November 1, 2005; 54(11): 3161 - 3168.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. N. Antonescu, C. Huang, W. Niu, Z. Liu, P. A. Eyers, K. A. Heidenreich, P. J. Bilan, and A. Klip
Reduction of Insulin-Stimulated Glucose Uptake in L6 Myotubes by the Protein Kinase Inhibitor SB203580 Is Independent of p38MAPK Activity
Endocrinology, September 1, 2005; 146(9): 3773 - 3781.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
N. Saravanan, A. Haseeb, N. Z Ehtesham, and Ghafoorunissa
Differential effects of dietary saturated and trans-fatty acids on expression of genes associated with insulin sensitivity in rat adipose tissue
Eur. J. Endocrinol., July 1, 2005; 153(1): 159 - 165.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Robidoux, W. Cao, H. Quan, K. W. Daniel, F. Moukdar, X. Bai, L. M. Floering, and S. Collins
Selective Activation of Mitogen-Activated Protein (MAP) Kinase Kinase 3 and p38{alpha} MAP Kinase Is Essential for Cyclic AMP-Dependent UCP1 Expression in Adipocytes
Mol. Cell. Biol., July 1, 2005; 25(13): 5466 - 5479.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Bazuine, F. Carlotti, M. J. W. E. Rabelink, J. Vellinga, R. C. Hoeben, and J. A. Maassen
The p38 Mitogen-Activated Protein Kinase Inhibitor SB203580 Reduces Glucose Turnover by the Glucose Transporter-4 of 3T3-L1 Adipocytes in the Insulin-Stimulated State
Endocrinology, April 1, 2005; 146(4): 1818 - 1824.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. C. Geiger, D. C. Wright, D.-H. Han, and J. O. Holloszy
Activation of p38 MAP kinase enhances sensitivity of muscle glucose transport to insulin
Am J Physiol Endocrinol Metab, April 1, 2005; 288(4): E782 - E788.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
E. Yamada, S. Okada, T. Saito, K. Ohshima, M. Sato, T. Tsuchiya, Y. Uehara, H. Shimizu, and M. Mori
Akt2 phosphorylates Synip to regulate docking and fusion of GLUT4-containing vesicles
J. Cell Biol., March 14, 2005; 168(6): 921 - 928.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
H. W.D. Yeung, R. K. Grewal, M. Gonen, H. Schoder, and S. M. Larson
Patterns of 18F-FDG Uptake in Adipose Tissue and Muscle: A Potential Source of False-Positives for PET
J. Nucl. Med., November 1, 2003; 44(11): 1789 - 1796.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Niu, C. Huang, Z. Nawaz, M. Levy, R. Somwar, D. Li, P. J. Bilan, and A. Klip
Maturation of the Regulation of GLUT4 Activity by p38 MAPK during L6 Cell Myogenesis
J. Biol. Chem., May 9, 2003; 278(20): 17953 - 17962.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
K. V. Kandror
A Long Search for Glut4 Activation
Sci. Signal., February 11, 2003; 2003(169): pe5 - pe5.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Somwar, S. Koterski, G. Sweeney, R. Sciotti, S. Djuric, C. Berg, J. Trevillyan, P. E. Scherer, C. M. Rondinone, and A. Klip
A Dominant-negative p38 MAPK Mutant and Novel Selective Inhibitors of p38 MAPK Reduce Insulin-stimulated Glucose Uptake in 3T3-L1 Adipocytes without Affecting GLUT4 Translocation
J. Biol. Chem., December 20, 2002; 277(52): 50386 - 50395.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2002 by the American Diabetes Association.