Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Larsen, M. O.
Right arrow Articles by Gotfredsen, C. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Larsen, M. O.
Right arrow Articles by Gotfredsen, C. F.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes 52:118-123, 2003
© 2003 by the American Diabetes Association, Inc.

Measurements of Insulin Secretory Capacity and Glucose Tolerance to Predict Pancreatic ß-Cell Mass In Vivo in the Nicotinamide/Streptozotocin Göttingen Minipig, a Model of Moderate Insulin Deficiency and Diabetes

Marianne O. Larsen1, Bidda Rolin1, Michael Wilken2, Richard D. Carr1, and Carsten F. Gotfredsen3

1 Department of Pharmacological Research I, Novo Nordisk A/S, Bagsvaerd, Denmark
2 Department of Assay and Cell Technology, Novo Nordisk A/S, Bagsvaerd, Denmark
3 Department of Histology, Novo Nordisk A/S, Bagsvaerd, Denmark

Knowledge about ß-cell mass and/or function could be of importance for the early diagnosis and treatment of diabetes. However, measurement of ß-cell function as an estimate of ß-cell mass is currently the only method possible in humans. The present study was performed to investigate different functional tests as predictors of ß-cell mass in the Göttingen minipig. ß-cell mass was reduced in the Göttingen minipig with a combination of nicotinamide (100 [n = 6], 67 [n = 25], 20 [n = 2], or 0 mg/kg [n = 4]) and streptozotocin (125 mg/kg). Six normal pigs were included. An oral glucose tolerance test (OGTT) (n = 43) and insulin secretion test (n = 30) were performed and pancreata obtained for stereological determination of ß-cell mass. During OGTT, fasting glucose (r2 = 0.1744, P < 0.01), area under the curve for glucose (r2 = 0.2706, P < 0.001), maximum insulin secretion (r2 = 0.2160, P < 0.01), and maximum C-peptide secretion (r2 = 0.1992, P < 0.01) correlated with ß-cell mass. During the insulin secretion test, acute insulin response to 0.3 g/kg (r2 = 0.6155, P < 0.0001) and 0.6 g/kg glucose (r2 = 0.7321, P < 0.0001) and arginine (67 mg/kg) (r2 = 0.7732, P < 0.0001) and maximum insulin secretion (r2 = 0.8192, P < 0.0001) correlated with ß-cell mass. This study supports the use of functional tests to evaluate ß-cell mass in vivo and has established a validated basis for developing a mathematical method for estimation of ß-cell mass in vivo in the Göttingen minipig.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DiabetesHome page
A. V. Matveyenko and P. C. Butler
{beta}-Cell Deficit Due to Increased Apoptosis in the Human Islet Amyloid Polypeptide Transgenic (HIP) Rat Recapitulates the Metabolic Defects Present in Type 2 Diabetes.
Diabetes, July 1, 2006; 55(7): 2106 - 2114.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. O. Larsen, B. Rolin, J. Sturis, M. Wilken, R. D. Carr, N. Porksen, and C. F. Gotfredsen
Measurements of insulin responses as predictive markers of pancreatic beta-cell mass in normal and beta-cell-reduced lean and obese Gottingen minipigs in vivo
Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E670 - E677.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. A. Ritzel, J. D. Veldhuis, and P. C. Butler
The mass, but not the frequency, of insulin secretory bursts in isolated human islets is entrained by oscillatory glucose exposure
Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E750 - E756.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. O. Larsen, C. B. Juhl, N. Porksen, C. F. Gotfredsen, R. D. Carr, U. Ribel, M. Wilken, and B. Rolin
{beta}-Cell function and islet morphology in normal, obese, and obese {beta}-cell mass-reduced Gottingen minipigs
Am J Physiol Endocrinol Metab, February 1, 2005; 288(2): E412 - E421.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. R. Rickels, M. H. Schutta, J. F. Markmann, C. F. Barker, A. Naji, and K. L. Teff
{beta}-Cell Function Following Human Islet Transplantation for Type 1 Diabetes
Diabetes, January 1, 2005; 54(1): 100 - 106.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. P. Robertson
Consequences on {beta}-Cell Function and Reserve After Long-Term Pancreas Transplantation
Diabetes, March 1, 2004; 53(3): 633 - 644.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. A. Ritzel, J. B. Hansen, J. D. Veldhuis, and P. C. Butler
Induction of {beta}-Cell Rest by a Kir6.2/SUR1-Selective KATP-Channel Opener Preserves {beta}-Cell Insulin Stores and Insulin Secretion in Human Islets Cultured at High (11 mM) Glucose
J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 795 - 805.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2003 by the American Diabetes Association.