HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Treutelaar, M. K. Articles by Burant, C. F. Search for Related Content PubMed PubMed Citation Articles by Treutelaar, M. K. Articles by Burant, C. F. Social Bookmarking                What's this?

Nestin-Lineage Cells Contribute to the Microvasculature but Not Endocrine Cells of the Islet

¹ Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan
² Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan
³ Department of Medicine, University of Chicago, Chicago, Illinois
⁴ Department of Surgery, University of Michigan, Ann Arbor, Michigan
⁵ Department of Human Genetics, University of Michigan, Ann Arbor, Michigan

To clarify the lineage relationship between cells that express the neural stem cell marker nestin and endocrine cells of the pancreas, we analyzed offspring of a cross between mice carrying a nestin promoter/enhancer-driven cre-recombinase (Nestin-cre) and C57BL/6J-Gtrosa26^tm1Sor mice that carry a loxP-disrupted ß-galactosidase gene (Rosa26). In nestin-cre^+/tg;R26R^loxP/+ embryos, cre-recombinase was detected in association with nestin-positive cells in the pancreatic mesenchyme with some of the nestin-positive cells lining vascular channels. In postnatal mice, pancreatic ß-galactosidase expression was restricted to vascular endothelial cells of the islet and a subset of cells in the muscularis of arteries in a distribution identical to endogenous nestin expression. Ex vivo explants of mouse pancreatic ducts grew dense cultures that costained for nestin and ß-galactosidase, demonstrating recombination in vitro. The cultures could be differentiated into complex stereotypic structures that contain nestin- and insulin-expressing cells. Nestin-cre^+/tg;R26R^loxP/+-derived duct cultures showed that insulin-positive cells were negative for ß-galactosidase. These results indicate that both in vivo and in vitro pancreatic endocrine cells arise independently of nestin-positive precursors. The apparent vascular nature of the nestin-positive cell population and the close association with endocrine cells suggest that nestin-positive cells play an important role in the growth and maintenance of the islet.

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Favaro, I. Miceli, B. Bussolati, M. Schimitt-Ney, P. Cavallo Perin, G. Camussi, and M. M. Zanone Hyperglycemia Induces Apoptosis of Human Pancreatic Islet Endothelial Cells: Effects of Pravastatin on the Akt Survival Pathway Am. J. Pathol., August 1, 2008; 173(2): 442 - 450. [Abstract] [Full Text] [PDF]

				N. Takahashi, M. T. Itoh, and B. Ishizuka Human Chorionic Gonadotropin Induces Nestin Expression in Endothelial Cells of the Ovary via Vascular Endothelial Growth Factor Signaling Endocrinology, January 1, 2008; 149(1): 253 - 260. [Abstract] [Full Text] [PDF]

				C. Carriere, E. S. Seeley, T. Goetze, D. S. Longnecker, and M. Korc The Nestin progenitor lineage is the compartment of origin for pancreatic intraepithelial neoplasia PNAS, March 13, 2007; 104(11): 4437 - 4442. [Abstract] [Full Text] [PDF]

				D. M. Simeone, L. Zhang, M. K. Treutelaar, L. Zhang, K. Graziano, C. D. Logsdon, and C. F. Burant Islet hypertrophy following pancreatic disruption of Smad4 signaling Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1305 - E1316. [Abstract] [Full Text] [PDF]

				X. Li, L. Zhang, S. Meshinchi, C. Dias-Leme, D. Raffin, J. D. Johnson, M. K. Treutelaar, and C. F. Burant Islet Microvasculature in Islet Hyperplasia and Failure in a Model of Type 2 Diabetes Diabetes, November 1, 2006; 55(11): 2965 - 2973. [Abstract] [Full Text] [PDF]

				A. Chamson-Reig, S. M Thyssen, E. Arany, and D. J Hill Altered pancreatic morphology in the offspring of pregnant rats given reduced dietary protein is time and gender specific. J. Endocrinol., October 1, 2006; 191(1): 83 - 92. [Abstract] [Full Text] [PDF]

				P. E. Forni, C. Scuoppo, I. Imayoshi, R. Taulli, W. Dastru, V. Sala, U. A. K. Betz, P. Muzzi, D. Martinuzzi, A. E. Vercelli, et al. High Levels of Cre Expression in Neuronal Progenitors Cause Defects in Brain Development Leading to Microencephaly and Hydrocephaly J. Neurosci., September 13, 2006; 26(37): 9593 - 9602. [Abstract] [Full Text] [PDF]

				M. Daimon, G. Ji, T. Oizumi, T. Kido, M. Baba, Y. Jimbu, W. Kameda, S. Susa, H. Yamaguchi, H. Ohnuma, et al. Association of Nephrin Gene Polymorphisms With Type 2 Diabetes in a Japanese Population: The Funagata Study Diabetes Care, May 1, 2006; 29(5): 1117 - 1119. [Full Text] [PDF]

				S. Thyssen, E. Arany, and D. J. Hill Ontogeny of Regeneration of {beta}-Cells in the Neonatal Rat after Treatment with Streptozotocin Endocrinology, May 1, 2006; 147(5): 2346 - 2356. [Abstract] [Full Text] [PDF]

				M. Hara, R. F. Dizon, B. S. Glick, C. S. Lee, K. H. Kaestner, D. W. Piston, and V. P. Bindokas Imaging pancreatic beta-cells in the intact pancreas Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E1041 - E1047. [Abstract] [Full Text] [PDF]

				M. E. Wilson, K. Y. Yang, A. Kalousova, J. Lau, Y. Kosaka, F. C. Lynn, J. Wang, C. Mrejen, V. Episkopou, H. C. Clevers, et al. The HMG Box Transcription Factor Sox4 Contributes to the Development of the Endocrine Pancreas Diabetes, December 1, 2005; 54(12): 3402 - 3409. [Abstract] [Full Text] [PDF]

				O. Krylyshkina, J. Chen, L. Mebis, C. Denef, and H. Vankelecom Nestin-Immunoreactive Cells in Rat Pituitary Are neither Hormonal nor Typical Folliculo-Stellate Cells Endocrinology, May 1, 2005; 146(5): 2376 - 2387. [Abstract] [Full Text] [PDF]

				R Wang, J Li, N Yashpal, and N Gao Nestin expression and clonal analysis of islet-derived epithelial monolayers: insight into nestin-expressing cell heterogeneity and differentiation potential J. Endocrinol., February 1, 2005; 184(2): 329 - 339. [Abstract] [Full Text] [PDF]

				S. S. Maria-Engler, M. L. C Correa-Giannella, L. Labriola, K. Krogh, C. Colin, F. H. Lojudice, C. A. M. Aita, E. M. C. de Oliveira, T. C S. Correa, I. C. da Silva, et al. Co-localization of nestin and insulin and expression of islet cell markers in long-term human pancreatic nestin-positive cell cultures J. Endocrinol., December 1, 2004; 183(3): 455 - 467. [Abstract] [Full Text] [PDF]

				Y. Choi, M. Ta, F. Atouf, and N. Lumelsky Adult Pancreas Generates Multipotent Stem Cells and Pancreatic and Nonpancreatic Progeny Stem Cells, November 1, 2004; 22(6): 1070 - 1084. [Abstract] [Full Text] [PDF]

				G. Shefer, M. Wleklinski-Lee, and Z. Yablonka-Reuveni Skeletal muscle satellite cells can spontaneously enter an alternative mesenchymal pathway J. Cell Sci., October 15, 2004; 117(22): 5393 - 5404. [Abstract] [Full Text] [PDF]

				E. A. Joanette, B. Reusens, E. Arany, S. Thyssen, R. C. Remacle, and D. J. Hill Low-Protein Diet during Early Life Causes a Reduction in the Frequency of Cells Immunopositive for Nestin and CD34 in Both Pancreatic Ducts and Islets in the Rat Endocrinology, June 1, 2004; 145(6): 3004 - 3013. [Abstract] [Full Text] [PDF]

				H. Segev, B. Fishman, A. Ziskind, M. Shulman, and J. Itskovitz-Eldor Differentiation of Human Embryonic Stem Cells into Insulin-Producing Clusters Stem Cells, May 1, 2004; 22(3): 265 - 274. [Abstract] [Full Text] [PDF]