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Contribution of Fatty Acids Released From Lipolysis of Plasma Triglycerides to Total Plasma Fatty Acid Flux and Tissue-Specific Fatty Acid Uptake

¹ TNO Prevention and Health, Gaubius Laboratory, Leiden, the Netherlands
² Department of Endocrinology, Leiden University Medical Center, Leiden, the Netherlands
³ Department of Cardiology and Internal Medicine, Leiden University Medical Center, Leiden, the Netherlands

There is controversy over the extent to which fatty acids (FAs) derived from plasma free FAs (FFAs) or from hydrolysis of plasma triglycerides (TGFAs) form communal or separate pools and what the contribution of each FA source is to cellular FA metabolism. Chylomicrons and lipid emulsions were labeled with [³H]triolein, injected into mice, and appearance in plasma of [³H]oleic acid was estimated, either through a steady-state approach or by compartmental modeling. [¹⁴C]oleic acid was included to trace plasma FFA. Eighty to 90% of triglyceride (TG) label was recovered in plasma, irrespective of tracer method or TG source. The contribution of TG lipolysis to total plasma FA turnover was 10–20%. After infusion of [³H]TG and [¹⁴C]FA, the retention of these labels varied substantially among liver, adipose tissue, and skeletal and heart muscle. Retention of TG label changed during fasting in the same direction as lipoprotein lipase (LPL) activity is regulated. We propose a model that reconciles the paradoxical 80–90% loss of TG label into plasma with LPL-directed differential uptake of TGFA in tissues. In this model, TGFAs mix locally at the capillaries with plasma FFAs, where they would lead to an increase in the local FA concentration, and hence, FA uptake. Our data indicate that a distinction between TG-derived FA and plasma FFA cannot be made.

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