Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, B.
Right arrow Articles by Harris, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, B.
Right arrow Articles by Harris, R. A.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes 52:1371-1376, 2003
© 2003 by the American Diabetes Association, Inc.

Starvation and Diabetes Reduce the Amount of Pyruvate Dehydrogenase Phosphatase in Rat Heart and Kidney

Boli Huang1, Pengfei Wu1, Kirill M. Popov2, and Robert A. Harris, 1

1 Department of Biochemistry and Molecular Biology, Indiana University and the School of Medicine, Indianapolis, Indiana
2 Division of Molecular Biology and Biochemistry, University of Missouri-Kansas City, Kansas City, Missouri

The pyruvate dehydrogenase complex (PDC) is inactivated in many tissues during starvation and diabetes to conserve three-carbon compounds for gluconeogenesis. This is achieved by an increase in the extent of PDC phosphorylation caused in part by increased pyruvate dehydrogenase kinase (PDK) activity due to increased PDK expression. This study examined whether altered pyruvate dehydrogenase phosphatase (PDP) expression also contributes to changes in the phosphorylation state of PDC during starvation and diabetes. Of the two PDP isoforms expressed in mammalian tissues, the Ca2+-sensitive isoform (PDP1) is highly expressed in rat heart, brain, and testis and is detectable but less abundant in rat muscle, lung, kidney, liver, and spleen. The Ca2+-insensitive isoform (PDP2) is abundant in rat kidney, liver, heart, and brain and is detectable in spleen and lung. Starvation and streptozotocin-induced diabetes cause decreases in PDP2 mRNA abundance, PDP2 protein amount, and PDP activity in rat heart and kidney. Refeeding and insulin treatment effectively reversed these effects of starvation and diabetes, respectively. These findings indicate that opposite changes in expression of specific PDK and PDP isoenzymes contribute to hyperphosphorylation and therefore inactivation of the PDC in heart and kidney during starvation and diabetes.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. Qi and B. Rodrigues
Glucocorticoids produce whole body insulin resistance with changes in cardiac metabolism
Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E654 - E667.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Li, X. Li, Y.-L. Li, C.-H. Shao, K. R. Bidasee, and G. J. Rozanski
Insulin regulation of glutathione and contractile phenotype in diabetic rat ventricular myocytes
Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1619 - H1629.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. Glyn-Jones, S. Song, M. A. Black, A. R. J. Phillips, S. Y. Choong, and G. J. S. Cooper
Transcriptomic analysis of the cardiac left ventricle in a rodent model of diabetic cardiomyopathy: molecular snapshot of a severe myocardial disease
Physiol Genomics, February 12, 2007; 28(3): 284 - 293.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. J. LeBlanc, R. A. Harris, and S. J. Peters
Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats
Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E571 - E576.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhang, K. Ma, P. Sadana, F. Chowdhury, S. Gaillard, F. Wang, D. P. McDonnell, T. G. Unterman, M. B. Elam, and E. A. Park
Estrogen-related Receptors Stimulate Pyruvate Dehydrogenase Kinase Isoform 4 Gene Expression
J. Biol. Chem., December 29, 2006; 281(52): 39897 - 39906.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. An and B. Rodrigues
Role of changes in cardiac metabolism in development of diabetic cardiomyopathy
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1489 - H1506.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. G. Korotchkina, S. Sidhu, and M. S. Patel
Characterization of Testis-specific Isoenzyme of Human Pyruvate Dehydrogenase
J. Biol. Chem., April 7, 2006; 281(14): 9688 - 9696.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Ma, Y. Zhang, M. B. Elam, G. A. Cook, and E. A. Park
Cloning of the Rat Pyruvate Dehydrogenase Kinase 4 Gene Promoter: ACTIVATION OF PYRUVATE DEHYDROGENASE KINASE 4 BY THE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR {gamma} COACTIVATOR
J. Biol. Chem., August 19, 2005; 280(33): 29525 - 29532.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. S. Golfman, C. R. Wilson, S. Sharma, M. Burgmaier, M. E. Young, P. H. Guthrie, M. Van Arsdall, J. V. Adrogue, K. K. Brown, and H. Taegtmeyer
Activation of PPAR{gamma} enhances myocardial glucose oxidation and improves contractile function in isolated working hearts of ZDF rats
Am J Physiol Endocrinol Metab, August 1, 2005; 289(2): E328 - E336.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. L. Pehleman, S. J. Peters, G. J. F. Heigenhauser, and L. L. Spriet
Enzymatic regulation of glucose disposal in human skeletal muscle after a high-fat, low-carbohydrate diet
J Appl Physiol, January 1, 2005; 98(1): 100 - 107.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. Qi, T. Pulinilkunnil, D. An, S. Ghosh, A. Abrahani, J. A. Pospisilik, R. Brownsey, R. Wambolt, M. Allard, and B. Rodrigues
Single-Dose Dexamethasone Induces Whole-Body Insulin Resistance and Alters Both Cardiac Fatty Acid and Carbohydrate Metabolism
Diabetes, July 1, 2004; 53(7): 1790 - 1797.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Li, P. Wu, P. Yarlagadda, N. M. Vadjunec, A. D. Proia, R. A. Harris, and D. Portilla
PPAR{alpha} ligand protects during cisplatin-induced acute renal failure by preventing inhibition of renal FAO and PDC activity
Am J Physiol Renal Physiol, March 1, 2004; 286(3): F572 - F580.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2003 by the American Diabetes Association.