|
 |
|
|||||||
|
|||||||||
Diabetes 52:1377-1385, 2003
© 2003 by the American Diabetes Association, Inc.
Differential Regulation of Protein Dynamics in Splanchnic and Skeletal Muscle Beds by Insulin and Amino Acids in Healthy Human Subjects
From the Division of Endocrinology, Mayo Clinic and Foundation, Rochester, Minnesota
To determine the in vivo effect of amino acids (AAs) alone or in combination with insulin on splanchnic and muscle protein dynamics, we infused stable isotope tracers of AAs in 36 healthy subjects and sampled from femoral artery and vein and hepatic vein. The subjects were randomized into six groups and were studied at baseline and during infusions of saline (group 1), insulin (0.5 mU · kg-1 · min-1) (group 2), insulin plus replacement of AAs (group 3) insulin plus high-dose AAs (group 4), or somatostatin and baseline replacement doses of insulin, glucagon and GH plus high dose of AAs (group 5) or saline (group 6). Insulin reduced muscle release of AAs mainly by inhibition of protein breakdown. Insulin also enhanced AA-induced muscle protein synthesis (PS) and reduced leucine transamination. The main effect of AAs on muscle was the enhancement of PS. Insulin had no effect on protein dynamics or leucine transamination in splanchnic bed. However, AAs reduced protein breakdown and increased synthesis in splanchnic bed in a dose-dependent manner. AAs also enhanced leucine transamination in both splanchnic and muscle beds. Thus insulin’s anabolic effect was mostly on muscle, whereas AAs acted on muscle as well as on splanchnic bed. Insulin achieved anabolic effect in muscle by inhibition of protein breakdown, enhancing AA-induced PS, and reducing leucine transamination. AAs largely determined protein anabolism in splanchnic bed by stimulating PS and decreasing protein breakdown.
CiteULike 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Pereira, E. B. Marliss, J. A. Morais, S. Chevalier, and R. Gougeon Insulin Resistance of Protein Metabolism in Type 2 Diabetes Diabetes, January 1, 2008; 57(1): 56 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bock, C. Dalla Man, M. Campioni, E. Chittilapilly, R. Basu, G. Toffolo, C. Cobelli, and R. Rizza Effects of Nonglucose Nutrients on Insulin Secretion and Action in People With Pre-Diabetes Diabetes, April 1, 2007; 56(4): 1113 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Chow, R. C. Albright, M. L. Bigelow, G. Toffolo, C. Cobelli, and K. S. Nair Mechanism of insulin's anabolic effect on muscle: measurements of muscle protein synthesis and breakdown using aminoacyl-tRNA and other surrogate measures Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E729 - E736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fujita, B. B. Rasmussen, J. G. Cadenas, J. J. Grady, and E. Volpi Effect of insulin on human skeletal muscle protein synthesis is modulated by insulin-induced changes in muscle blood flow and amino acid availability Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E745 - E754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Bell, E. Volpi, S. Fujita, J. G. Cadenas, M. Sheffield-Moore, and B. B. Rasmussen Skeletal Muscle Protein Anabolic Response to Increased Energy and Insulin Is Preserved in Poorly Controlled Type 2 Diabetes J. Nutr., May 1, 2006; 136(5): 1249 - 1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Prod'homme, C. Rochon, M. Balage, H. Laurichesse, I. Tauveron, C. Champredon, P. Thieblot, J. Beytout, and J. Grizard Whole body leucine flux in HIV-infected patients treated with or without protease inhibitors Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E685 - E693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fujita and E. Volpi Amino Acids and Muscle Loss with Aging J. Nutr., January 1, 2006; 136(1): 277S - 280S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tom and K. S. Nair Assessment of Branched-Chain Amino Acid Status and Potential for Biomarkers J. Nutr., January 1, 2006; 136(1): 324S - 330S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Bell, S. Fujita, E. Volpi, J. G. Cadenas, and B. B. Rasmussen Short-term insulin and nutritional energy provision do not stimulate muscle protein synthesis if blood amino acid availability decreases Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E999 - E1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Nair and K. R. Short Hormonal and Signaling Role of Branched-Chain Amino Acids J. Nutr., June 1, 2005; 135(6): 1547S - 1552S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K S. Nair Aging muscle Am. J. Clinical Nutrition, May 1, 2005; 81(5): 953 - 963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Geukers, J. H Oudshoorn, J. A. Taminiau, C. K van der Ent, P. Schilte, A. F. Ruiter, M. T Ackermans, E. Endert, C. F Jonkers-Schuitema, H. S. Heymans, et al. Short-term protein intake and stimulation of protein synthesis in stunted children with cystic fibrosis Am. J. Clinical Nutrition, March 1, 2005; 81(3): 605 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B Mittendorfer, J. L Andersen, P Plomgaard, B Saltin, J. A Babraj, K Smith, and M. J Rennie Protein synthesis rates in human muscles: neither anatomical location nor fibre-type composition are major determinants J. Physiol., February 15, 2005; 563(1): 203 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jaleel and K. S. Nair Identification of multiple proteins whose synthetic rates are enhanced by high amino acid levels in rat hepatocytes Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E950 - E957. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Diabetes | Diabetes Care | Clinical Diabetes | Diabetes Spectrum |