HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kowluru, R. A. Articles by Odenbach, S. Search for Related Content PubMed PubMed Citation Articles by Kowluru, R. A. Articles by Odenbach, S. Social Bookmarking                What's this?

Effect of Long-Term Administration of -Lipoic Acid on Retinal Capillary Cell Death and the Development of Retinopathy in Diabetic Rats

From the Kresge Eye Institute, Wayne State University, Detroit, Michigan

Oxidative stress is increased in the retina in diabetes, and it is considered to play an important role in the development of retinopathy. -Lipoic acid, a thiol antioxidant, has been shown to have beneficial effects on polyneuropathy and on the parameters of oxidative stress in various tissues, including nerve, kidney, and retina. The purpose of this study was to examine the effect of -lipoic acid on retinal capillary cell apoptosis and the development of pathology in diabetes. Retina was used from streptozotocin-induced diabetic rats receiving diets supplemented with or without -lipoic acid (400 mg/kg) for 11 months of diabetes. Capillary cell apoptosis (by terminal transferase-mediated dUTP nick-end labeling) and formation of acellular capillaries were investigated in the trypsin-digested retinal microvessels. The effect of -lipoic acid administration on retinal 8-hydroxy-2'deoxyguanosine (8-OHdG) and nitrotyrosine levels was determined by enzyme-linked immunosorbent assay. -Lipoic acid administration for the entire duration of diabetes inhibited capillary cell apoptosis and the number of acellular capillaries in the retina, despite similar severity of hyperglycemia in the two diabetic groups (with and without -lipoic acid). Retinal 8-OHdG and nitrotyrosine levels were increased by over twofold and 70%, respectively, in diabetes, and -lipoic acid administration inhibited these increases. Our results demonstrate that the long-term administration of -lipoic acid has beneficial effects on the development of diabetic retinopathy via inhibition of accumulation of oxidatively modified DNA and nitrotyrosine in the retina. -Lipoic acid supplementation represents an achievable adjunct therapy to help prevent vision loss in diabetic patients.

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. A. Kowluru, M. Kanwar, P.-S. Chan, and J. P. Zhang Inhibition of Retinopathy and Retinal Metabolic Abnormalities in Diabetic Rats With AREDS-Based Micronutrients Arch Ophthalmol, September 1, 2008; 126(9): 1266 - 1272. [Abstract] [Full Text] [PDF]

				F. Pfister, Y. Feng, F. vom Hagen, S. Hoffmann, G. Molema, J.-L. Hillebrands, M. Shani, U. Deutsch, and H.-P. Hammes Pericyte Migration: A Novel Mechanism of Pericyte Loss in Experimental Diabetic Retinopathy Diabetes, September 1, 2008; 57(9): 2495 - 2502. [Abstract] [Full Text] [PDF]

				B. A. Berkowitz, R. Roberts, A. Stemmler, H. Luan, and M. Gradianu Impaired Apparent Ion Demand in Experimental Diabetic Retinopathy: Correction by Lipoic Acid Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4753 - 4758. [Abstract] [Full Text] [PDF]

				Y. Ishida, T. Ohara, Y. Okuno, T. Ito, Y. Hirota, K. Furukawa, K. Sakaguchi, W. Ogawa, and M. Kasuga {alpha}-Lipoic Acid and Insulin Autoimmune Syndrome Diabetes Care, September 1, 2007; 30(9): 2240 - 2241. [Full Text] [PDF]

				M. Artwohl, K. Muth, K. Kosulin, R. de Martin, T. Holzenbein, G. Rainer, A. Freudenthaler, N. Huttary, L. Schmetterer, W. K. Waldhausl, et al. R-(+)-{alpha}-lipoic acid inhibits endothelial cell apoptosis and proliferation: involvement of Akt and retinoblastoma protein/E2F-1 Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E681 - E689. [Abstract] [Full Text] [PDF]

				P. Larghero, R. Vene, S. Minghelli, G. Travaini, M. Morini, N. Ferrari, U. Pfeffer, D. M. Noonan, A. Albini, and R. Benelli Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression Carcinogenesis, May 1, 2007; 28(5): 1008 - 1020. [Abstract] [Full Text] [PDF]

				J. A. Phipps, J. L. Wilkinson-Berka, and E. L. Fletcher Retinal Dysfunction in Diabetic Ren-2 Rats Is Ameliorated by Treatment with Valsartan but Not Atenolol Invest. Ophthalmol. Vis. Sci., February 1, 2007; 48(2): 927 - 934. [Abstract] [Full Text] [PDF]

				R. Roberts, H. Luan, and B. A. Berkowitz {alpha}-Lipoic Acid Corrects Late-Phase Supernormal Retinal Oxygenation Response in Experimental Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 4077 - 4082. [Abstract] [Full Text] [PDF]

				K. Komeima, B. S. Rogers, L. Lu, and P. A. Campochiaro Antioxidants reduce cone cell death in a model of retinitis pigmentosa PNAS, July 25, 2006; 103(30): 11300 - 11305. [Abstract] [Full Text] [PDF]

				R. A. Kowluru, L. Atasi, and Y.-S. Ho Role of mitochondrial superoxide dismutase in the development of diabetic retinopathy. Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1594 - 1599. [Abstract] [Full Text] [PDF]