HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, L. Articles by Barrett, E. J. Search for Related Content PubMed PubMed Citation Articles by Zhang, L. Articles by Barrett, E. J. Social Bookmarking                What's this?

Insulin Sensitivity of Muscle Capillary Recruitment In Vivo

¹ Department of Biochemistry, University of Tasmania, Hobart, Tasmania, Australia
² Department of Internal Medicine, University of Virginia, Charlottesville, Virginia

We have reported that insulin exerts two vascular actions in muscle; it both increases blood flow and recruits capillaries. In parallel hyperinsulinemic-euglycemic clamp studies, we compared the insulin dose response of muscle microvascular recruitment and femoral blood flow as well as hindleg glucose uptake in fed, hooded Wistar and fasted Sprague-Dawley rats. Using insulin doses between 0 and 30 mU^-1 · min^-1 · kg^-1, we measured microvascular recruitment at 2 h by 1-methylxanthine (1-MX) metabolism or contrast-enhanced ultrasound (CEU), and muscle glucose uptake was measured by either arteriovenous differences or using 2-deoxyglucose. We also examined the time course for reversal of microvascular recruitment following cessation of a 3 mU · min^-1 · kg^-1 insulin infusion. In both groups, whether measured by 1-MX metabolism or CEU, microvascular recruitment was fully activated by physiologic hyperinsulinemia and occurred at lower insulin concentrations than those that stimulated glucose uptake or hindleg total blood flow. The latter processes were insulin dose dependent throughout the entire dose range studied. Upon stopping the insulin infusion, increases in microvascular volume persisted for 15–30 min after insulin concentrations returned to basal levels. We conclude that the precapillary arterioles that regulate microvascular recruitment are more insulin sensitive than resistance arterioles that regulate total flow.

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Wang, A. X. Wang, Z. Liu, and E. J. Barrett Insulin Signaling Stimulates Insulin Transport by Bovine Aortic Endothelial Cells Diabetes, March 1, 2008; 57(3): 540 - 547. [Abstract] [Full Text] [PDF]

				E. M. Eggleston, L. A. Jahn, and E. J. Barrett Hyperinsulinemia Rapidly Increases Human Muscle Microvascular Perfusion but Fails to Increase Muscle Insulin Clearance: Evidence That a Saturable Process Mediates Muscle Insulin Uptake Diabetes, December 1, 2007; 56(12): 2958 - 2963. [Abstract] [Full Text] [PDF]

				J. M. B. Newman, R. M. Ross, S. M. Richards, M. G. Clark, and S. Rattigan Insulin and contraction increase nutritive blood flow in rat muscle in vivo determined by microdialysis of L-[14C]glucose J. Physiol., November 15, 2007; 585(1): 217 - 229. [Abstract] [Full Text] [PDF]

				A. M. Jonk, A. J. H. M. Houben, R. T. de Jongh, E. H. Serne, N. C. Schaper, and C. D. A. Stehouwer Microvascular Dysfunction in Obesity: A Potential Mechanism in the Pathogenesis of Obesity-Associated Insulin Resistance and Hypertension Physiology, August 1, 2007; 22(4): 252 - 260. [Abstract] [Full Text] [PDF]

				R. Muniyappa, M. Montagnani, K. K. Koh, and M. J. Quon Cardiovascular Actions of Insulin Endocr. Rev., August 1, 2007; 28(5): 463 - 491. [Abstract] [Full Text] [PDF]

				W. Chai and Z. Liu p38 Mitogen-Activated Protein Kinase Mediates Palmitate-Induced Apoptosis But Not Inhibitor of Nuclear Factor-{kappa}B Degradation in Human Coronary Artery Endothelial Cells Endocrinology, April 1, 2007; 148(4): 1622 - 1628. [Abstract] [Full Text] [PDF]

				J. E. Ayala, D. P. Bracy, B. M. Julien, J. N. Rottman, P. T. Fueger, and D. H. Wasserman Chronic Treatment With Sildenafil Improves Energy Balance and Insulin Action in High Fat-Fed Conscious Mice Diabetes, April 1, 2007; 56(4): 1025 - 1033. [Abstract] [Full Text] [PDF]

				E. A. Bradley, M. G. Clark, and S. Rattigan Acute effects of wortmannin on insulin's hemodynamic and metabolic actions in vivo Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E779 - E787. [Abstract] [Full Text] [PDF]

				V. Wong, L. Szeto, K. Uffelman, I G. Fantus, and G. F Lewis Enhancement of muscle glucose uptake by the vasopeptidase inhibitor, omapatrilat, is independent of insulin signaling and the AMP kinase pathway. J. Endocrinol., August 1, 2006; 190(2): 441 - 450. [Abstract] [Full Text] [PDF]

				H. Wang, Z. Liu, G. Li, and E. J. Barrett The vascular endothelial cell mediates insulin transport into skeletal muscle Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E323 - E332. [Abstract] [Full Text] [PDF]

				M. A. Vincent, L. H. Clerk, J. R. Lindner, W. J. Price, L. A. Jahn, H. Leong-Poi, and E. J. Barrett Mixed meal and light exercise each recruit muscle capillaries in healthy humans Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1191 - E1197. [Abstract] [Full Text] [PDF]

				L. H. Clerk, M. A. Vincent, L. A. Jahn, Z. Liu, J. R. Lindner, and E. J. Barrett Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle. Diabetes, May 1, 2006; 55(5): 1436 - 1442. [Abstract] [Full Text] [PDF]

				J.-a Kim, M. Montagnani, K. K. Koh, and M. J. Quon Reciprocal Relationships Between Insulin Resistance and Endothelial Dysfunction: Molecular and Pathophysiological Mechanisms Circulation, April 18, 2006; 113(15): 1888 - 1904. [Abstract] [Full Text] [PDF]

				A. J.M. Rennings, P. Smits, M. W. Stewart, and C. J. Tack Fluid retention and vascular effects of rosiglitazone in obese, insulin-resistant, nondiabetic subjects. Diabetes Care, March 1, 2006; 29(3): 581 - 587. [Abstract] [Full Text] [PDF]

				G. Li, E. J. Barrett, H. Wang, W. Chai, and Z. Liu Insulin at Physiological Concentrations Selectively Activates Insulin But Not Insulin-Like Growth Factor I (IGF-I) or Insulin/IGF-I Hybrid Receptors in Endothelial Cells Endocrinology, November 1, 2005; 146(11): 4690 - 4696. [Abstract] [Full Text] [PDF]

				R. Scognamiglio, C. Negut, S. V. De Kreutzenberg, A. Tiengo, and A. Avogaro Postprandial Myocardial Perfusion in Healthy Subjects and in Type 2 Diabetic Patients Circulation, July 12, 2005; 112(2): 179 - 184. [Abstract] [Full Text] [PDF]

				D. B. Olsen, M. Sacchetti, F. Dela, T. Ploug, and B. Saltin Glucose clearance is higher in arm than leg muscle in type 2 diabetes J. Physiol., June 1, 2005; 565(2): 555 - 562. [Abstract] [Full Text] [PDF]

				S. Gudbjornsdottir, M. Sjostrand, L. Strindberg, and P. Lonnroth Decreased Muscle Capillary Permeability Surface Area in Type 2 Diabetic Subjects J. Clin. Endocrinol. Metab., February 1, 2005; 90(2): 1078 - 1082. [Abstract] [Full Text] [PDF]

				R. T. de Jongh, E. H. Serne, R. G. IJzerman, G. de Vries, and C. D.A. Stehouwer Free Fatty Acid Levels Modulate Microvascular Function: Relevance for Obesity-Associated Insulin Resistance, Hypertension, and Microangiopathy Diabetes, November 1, 2004; 53(11): 2873 - 2882. [Abstract] [Full Text] [PDF]

				C. M. Wheatley, S. Rattigan, S. M. Richards, E. J. Barrett, and M. G. Clark Skeletal muscle contraction stimulates capillary recruitment and glucose uptake in insulin-resistant obese Zucker rats Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E804 - E809. [Abstract] [Full Text] [PDF]