|
 |
|
|||||||
|
|||||||||
Diabetes 53:752-761, 2004
© 2004 by the American Diabetes Association, Inc.
Hyperglycemia-Induced Defects in Angiogenesis in the Chicken Chorioallantoic Membrane Model
1 INSERM U36, Chaire de Médecine Expérimentale, Collège de France, Paris, France
2 Faculté de Médecine X, Bichat, Université Paris 7, Paris, France
Diabetes is associated with abnormal angiogenesis. Increased angiogenesis contributes to severe forms of diabetic retinopathy, but angiogenesis is decreased in response to myocardial ischemia in diabetic patients. We evaluated the direct effect of hyperglycemia on angiogenesis in the chicken chorioallantoic membrane assay, a model of active neoangiogenesis. Hyperglycemia, lasting up to 7 days, was induced in 7-day-old chick embryos by a single intravitellus glucose injection. Control embryos received either water (volumic control) or mannitol (osmotic control). Hyperglycemia decreased angiogenesis in this model from the 5th day on. The pattern and expression level of the main vascular growth factors’ genes were not altered by hyperglycemia, as assessed by in situ hybridization and semiquantitative RT-PCR. As early as 2 days after hyperglycemia was induced, an increased apoptosis of endothelial cells and pericytes was detected by transferase-mediated deoxyuridine triphosphate nick-end labeling assay and electron microscopy. In the meantime, endothelial cell proliferation was decreased, as assessed by incorporation of bromo-deoxyuridine. Hyperglycemia can therefore impair angiogenesis without altering the expression level of vascular growth factors through induction of apoptosis and decreased proliferation of endothelial cells.
Address correspondence and reprint requests to Etienne Larger, MD, INSERM U36, Collège de France, 11 pl. M. Berthelot, F-75005 Paris, France. E-mail: etienne.larger{at}college-de-france.fr
CiteULike 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Facchiano, D. D'Arcangelo, K. Russo, V. Fogliano, C. Mennella, R. Ragone, G. Zambruno, V. Carbone, D. Ribatti, C. Peschle, et al. Glycated Fibroblast Growth Factor-2 Is Quickly Produced in Vitro upon Low-Millimolar Glucose Treatment and Detected in Vivo in Diabetic Mice Mol. Endocrinol., November 1, 2006; 20(11): 2806 - 2818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-S. Fetita, E. Sobngwi, P. Serradas, F. Calvo, and J.-F. Gautier Consequences of Fetal Exposure to Maternal Diabetes in Offspring J. Clin. Endocrinol. Metab., October 1, 2006; 91(10): 3718 - 3724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shoji, H. Koyama, T. Morioka, S. Tanaka, A. Kizu, K. Motoyama, K. Mori, S. Fukumoto, A. Shioi, N. Shimogaito, et al. Receptor for advanced glycation end products is involved in impaired angiogenic response in diabetes. Diabetes, August 1, 2006; 55(8): 2245 - 2255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kivela, M. Silvennoinen, A.-M. Touvra, T. M. Lehti, H. Kainulainen, and V. Vihko Effects of experimental type 1 diabetes and exercise training on angiogenic gene expression and capillarization in skeletal muscle FASEB J, July 1, 2006; 20(9): 1570 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Pardanaud and A. Eichmann Identification, emergence and mobilization of circulating endothelial cells or progenitors in the embryo Development, July 1, 2006; 133(13): 2527 - 2537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Savary, A. Michaud, J. Favier, E. Larger, P. Corvol, and J.-M. Gasc Role of the renin-angiotensin system in primitive erythropoiesis in the chick embryo Blood, January 1, 2005; 105(1): 103 - 110. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Diabetes | Diabetes Care | Clinical Diabetes | Diabetes Spectrum |