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53.06.04.db04-0174v1
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Diabetes 53:1407-1411, 2004
© 2004 by the American Diabetes Association, Inc.

Rapid Publication

Human Pancreatic Duct Cells Exert Tissue Factor-Dependent Procoagulant Activity

Relevance to Islet Transplantation

Claire Beuneu1,2, Olivier Vosters1,2, Babak Movahedi2,3, Myriam Remmelink4, Isabelle Salmon4, Daniel Pipeleers2,3, Olivier Pradier1, Michel Goldman1,2, and Valérie Verhasselt1,2

1 Laboratory of Experimental Immunology and Institute for Medical Immunology, Université Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium
2 Juvenile Diabetes Research Foundation Center for ß-Cell Therapy in Europe, Brussels, Belgium
3 Diabetes Research Center, Vrije Universiteit Brussels-VUB, Brussels, Belgium
4 Laboratory of Pathology, Université Libre de Bruxelles-ULB, Erasme Hospital, Brussels, Belgium

Activation of the coagulation cascade contributes to early graft loss and intraportal thrombotic events in clinical islet transplantation. Although these complications were shown to be related to the presence of tissue factor in human islet preparations, the contribution of duct cells, which represent a major contaminant of clinical islet isolates, has not been specified so far. Herein, we used flow cytometry, immunohistochemistry, RT-PCR, and functional coagulation assays to demonstrate that duct cells exert a potent factor VII-dependent procoagulant activity related to their expression of tissue factor. Both the classical membrane-bound and the recently described soluble form of tissue factor were shown to be synthesized by duct cells. We conclude that contaminating duct cells contribute to early ß-cell damage after islet transplantation through their involvement in tissue factor-mediated thrombotic and inflammatory events.

Address correspondence and reprint requests to Michel Goldman, MD, PhD, Laboratory of Experimental Immunology, Université Libre de Bruxelles, Hôpital Erasme, 808 Route de Lennik, B-1070 Brussels, Belgium. E-mail: mgoldman{at}ulb.ac.be


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Copyright © 2004 by the American Diabetes Association.