HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schoelch, C. Articles by Herling, A. W. Search for Related Content PubMed PubMed Citation Articles by Schoelch, C. Articles by Herling, A. W. Social Bookmarking                What's this?

Characterization of Adenosine-A1 Receptor–Mediated Antilipolysis in Rats by Tissue Microdialysis, ¹H-Spectroscopy, and Glucose Clamp Studies

From Aventis Pharma Deutschland, Frankfurt am Main, Germany

Increased supply of fatty acids to muscle and liver is causally involved in the insulin resistance syndrome. Using a tissue microdialysis technique in Wistar and Zucker fatty (ZF) rats, we determined tissue glycerol levels as a marker of lipolysis in gastrocnemius muscle (gMT), subcutaneous adipose (SAT), and visceral adipose tissue (VAT) as well as the reduction of plasma free fatty acids, glycerol, and triglycerides caused by the antilipolysis-specific adenosine-A1 receptor agonist (ARA). In Wistar and ZF rats, ARA significantly lowered dialysate glycerol levels in SAT, VAT, and gMT. Whereas in SAT and VAT the decrease in dialysate glycerol indicated adipocytic antilipolysis, this decrease in gMT was not caused by a direct effect of ARA on intramyocellular lipolysis, as demonstrated by the lack of inhibition of the protein kinase A activity ratio in gMT. In addition, no differences of the fed-starved-refed dynamics of intramyocellular triglyceride levels compared with untreated controls were measured by in vivo ¹H-spectroscopy, excluding any adenylate cyclase–independent antilipolysis in muscle. Treatment with ARA resulted in pronounced reductions of plasma free fatty acids, glycerol, and triglycerides. Furthermore, in ZF rats, ARA treatment caused an immediate improvement of peripheral insulin sensitivity measured by the euglycemic-hyperinsulinemic glucose clamp technique.

Abbreviations: ARA, adenosine-A1 receptor agonist; FFA, free fatty acid; GIR, glucose infusion rate; gMT, gastrocnemius muscle tissue; HSL, hormone-sensitive lipase; IMTG, intramyocellular TG; mLPL, muscle lipoprotein lipase; MRS, magnetic resonance spectroscopy; PKA, protein kinase A; SAT, subcutaneous adipose tissue; SOL, m. soleus; TG, triglyceride; TIB, m. tibialis; VAT, visceral adipose tissue

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. K. Dhalla, M. Santikul, M. Smith, M.-Y. Wong, J. C. Shryock, and L. Belardinelli Antilipolytic Activity of a Novel Partial A1 Adenosine Receptor Agonist Devoid of Cardiovascular Effects: Comparison with Nicotinic Acid J. Pharmacol. Exp. Ther., April 1, 2007; 321(1): 327 - 333. [Abstract] [Full Text] [PDF]

				H. Barakat, J. Davis, D. Lang, S. J. Mustafa, and M. M. McConnaughey Differences in the Expression of the Adenosine A1 Receptor in Adipose Tissue of Obese Black and White Women J. Clin. Endocrinol. Metab., May 1, 2006; 91(5): 1882 - 1886. [Abstract] [Full Text] [PDF]