HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fujita, S. Articles by Donovan, C. M. Search for Related Content PubMed PubMed Citation Articles by Fujita, S. Articles by Donovan, C. M. Social Bookmarking                What's this?

Celiac-Superior Mesenteric Ganglionectomy, but Not Vagotomy, Suppresses the Sympathoadrenal Response to Insulin-Induced Hypoglycemia

Departments of Kinesiology and Integrative and Evolutionary Biology, University of Southern California, Los Angeles, California

Afferent innervation of the portal vein has been shown to be critical in hypoglycemic detection, but the neural pathway by which these afferents ascend remains unknown. To ascertain the role of vagal afferents versus spinal afferents in hypoglycemic detection, the catecholamine response to hypoglycemia was assessed in male Wistar rats undergoing hepatic vagotomy (HV), total subdiaphragmatic vagotomy (TSV), or celiac-superior mesenteric ganglionectomy (CSMG). After recovering from the surgery, the animals were exposed to a hyperinsulinemic-hypoglycemic clamp, with glucose infused peripherally via the jugular vein. In all animals, systemic hypoglycemia (2.64 ± 0.03 mmol/l) was induced via jugular vein insulin infusion (25 mU · kg^–1 · min^–1). No significant differences were observed among the groups with respect to arterial glucose or insulin concentration. When hypoglycemia was induced in sham-operated control animals, epinephrine was observed to rise from a basal value of 0.84 ± 0.10 to 25.18 ± 1.24 nmol/l. Neither HV nor TSV had any significant impact on the epinephrine response to hypoglycemia. In contrast, CSMG animals demonstrated a significant suppression in the epinephrine response to whole-body hypoglycemia (11.25 ± 1.21 vs. 22.32 ± 0.86 nmol/l in CSMG vs. controls; P < 0.05). The norepinephrine response for controls, 2.00 ± 0.22 at basal and rising to 8.95 ± 0.20 nmol/l in hypoglycemia, was not significantly different from that of the HV and TSV animals. As with epinephrine, the norepinephrine response to hypoglycemia was significantly suppressed in CSMG compared with control animals (4.72 ± 0.48 vs. 7.15 ± 0.76 nmol/l; P < 0.05). These findings are consistent with the idea that hypoglycemic detection at the portal vein is mediated by spinal, and not vagal, glucose-sensitive afferents.

Key Words: 2-DG, 2-deoxyglucose • CGRP, calcitonin gene-related peptide • CSMG, celiac-superior mesenteric ganglionectomy • HV, hepatic vagotomy • TSV, total subdiaphragmatic vagotomy

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. D. Cherrington Central Versus Peripheral Glucose Sensing and the Response to Hypoglycemia Diabetes, May 1, 2008; 57(5): 1158 - 1159. [Full Text] [PDF]

				M. Saberi, M. Bohland, and C. M. Donovan The Locus for Hypoglycemic Detection Shifts With the Rate of Fall in Glycemia: The Role of Portal-Superior Mesenteric Vein Glucose Sensing Diabetes, May 1, 2008; 57(5): 1380 - 1386. [Abstract] [Full Text] [PDF]

				A. V. Matveyenko, M. Bohland, M. Saberi, and C. M. Donovan Portal vein hypoglycemia is essential for full induction of hypoglycemia-associated autonomic failure with slow-onset hypoglycemia Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E857 - E864. [Abstract] [Full Text] [PDF]

				M. Fukaya, A. Mizuno, H. Arai, K. Muto, T. Uebanso, K. Matsuo, H. Yamamoto, Y. Taketani, T. Doi, and E. Takeda Mechanism of rapid-phase insulin response to elevation of portal glucose concentration Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E515 - E522. [Abstract] [Full Text] [PDF]

				S. Fujita, M. Bohland, G. Sanchez-Watts, A. G. Watts, and C. M. Donovan Hypoglycemic detection at the portal vein is mediated by capsaicin-sensitive primary sensory neurons Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E96 - E101. [Abstract] [Full Text] [PDF]

				B. E. Levin, L. Kang, N. M. Sanders, and A. A. Dunn-Meynell Role of Neuronal Glucosensing in the Regulation of Energy Homeostasis Diabetes, December 1, 2006; 55(Supplement_2): S122 - S130. [Abstract] [Full Text] [PDF]

				A. V. Matveyenko and C. M. Donovan Metabolic sensors mediate hypoglycemic detection at the portal vein. Diabetes, May 1, 2006; 55(5): 1276 - 1282. [Abstract] [Full Text] [PDF]