HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Satoh, H. Articles by Olefsky, J. M. Search for Related Content PubMed PubMed Citation Articles by Satoh, H. Articles by Olefsky, J. M. Social Bookmarking                What's this?

Adenovirus-Mediated Adiponectin Expression Augments Skeletal Muscle Insulin Sensitivity in Male Wistar Rats

¹ Department of Medicine, Division of Endocrinology and Metabolism, University of California-San Diego, La Jolla, California
² Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York
³ Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, New York
⁴ San Diego Veterans Affairs Medical Center, La Jolla, California
⁵ Larry L. Hillblom Foundation, La Jolla, California

In this study, we investigated the chronic in vivo effect of adiponectin on insulin sensitivity and glucose metabolism by overexpressing the adiponectin protein in male Wistar rats using intravenous administration of an adenovirus (Adv-Adipo). Virally infected liver secreted adiponectin as high and low molecular weight complexes. After 7 days of physiological or supraphysiological hyperadiponectinemia, the animals displayed enhanced insulin sensitivity during the glucose tolerance and insulin tolerance tests. Glucose clamp studies performed at submaximal and maximal insulin infusion rates (4 and 25 mU · kg^–1 · min^–1, respectively) also demonstrated increased insulin sensitivity in Adv-Adipo animals, with the insulin-stimulated glucose disposal rate being increased by 20–67%. In contrast, insulin’s effect on the suppression of hepatic glucose output and plasma free fatty acid levels was not enhanced in Adv-Adipo rats compared with controls, suggesting that high levels of adiponectin expression in the liver may lead to a local desensitization. Consistent with the clamp data, the activation of AMP-activated protein kinase was significantly enhanced in skeletal muscle (by 50%) but not in liver. One interesting finding was that in male Wistar rats, both AdipoR1 and AdipoR2 expression levels were higher in skeletal muscle than in liver, as it is the case in humans. These results indicate that chronic adiponectin treatment enhances insulin sensitivity and could serve as a therapy for human insulin resistance.

Abbreviations: Adv, adenovirus; AMPK, AMP-activated protein kinase; ELISA, enzyme-linked immunosorbent assay; FFA, free fatty acid; GDR, glucose disposal rate; GINF, glucose infusion rate; GTT, glucose tolerance test; HF, high fat; HGO, hepatic glucose output; HMW, high molecular weight; IR, insulin receptor; IRS, insulin receptor substrate; ISGDR, insulin-stimulated GDR; ITT, insulin tolerance test; LMW, low molecular weight; RIA, radioimmunoassay; TZD, thiazolidinedione

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Caglayan, B. Stauber, A. R. Collins, C. J. Lyon, F. Yin, J. Liu, S. Rosenkranz, E. Erdmann, L. E. Peterson, R. S. Ross, et al. Differential Roles of Cardiomyocyte and Macrophage Peroxisome Proliferator-Activated Receptor {gamma} in Cardiac Fibrosis Diabetes, September 1, 2008; 57(9): 2470 - 2479. [Abstract] [Full Text] [PDF]

				L. Qiao, C. Zou, D. R. van der Westhuyzen, and J. Shao Adiponectin Reduces Plasma Triglyceride by Increasing VLDL Triglyceride Catabolism Diabetes, July 1, 2008; 57(7): 1824 - 1833. [Abstract] [Full Text] [PDF]

				C.-C. Juan, T.-Y. Chuang, C.-L. Chang, S.-W. Huang, and L.-T. Ho Endothelin-1 Regulates Adiponectin Gene Expression and Secretion in 3T3-L1 Adipocytes via Distinct Signaling Pathways Endocrinology, April 1, 2007; 148(4): 1835 - 1842. [Abstract] [Full Text] [PDF]

				J. V. Silha, B.L. G. Nyomba, W. D. Leslie, and L. J. Murphy Ethnicity, Insulin Resistance, and Inflammatory Adipokines in Women at High and Low Risk for Vascular Disease Diabetes Care, February 1, 2007; 30(2): 286 - 291. [Abstract] [Full Text] [PDF]

				N. Pannacciulli, J. C. Bunt, E. Ortega, T. Funahashi, A. D. Salbe, C. Bogardus, and J. Krakoff Lower Total Fasting Plasma Adiponectin Concentrations Are Associated with Higher Metabolic Rates J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1600 - 1603. [Abstract] [Full Text] [PDF]

				O. J. Martin, A. Lee, and T. E. McGraw GLUT4 Distribution between the Plasma Membrane and the Intracellular Compartments Is Maintained by an Insulin-modulated Bipartite Dynamic Mechanism J. Biol. Chem., January 6, 2006; 281(1): 484 - 490. [Abstract] [Full Text] [PDF]

				J. J. Wilkes, M. T. A. Nguyen, G. K. Bandyopadhyay, E. Nelson, and J. M. Olefsky Topiramate treatment causes skeletal muscle insulin sensitization and increased Acrp30 secretion in high-fat-fed male Wistar rats Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1015 - E1022. [Abstract] [Full Text] [PDF]