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Functional Defects and the Influence of Age on the Frequency of CD4⁺CD25⁺ T-Cells in Type 1 Diabetes

¹ Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, Florida
² Department of Pediatrics, University of Florida, Gainesville, Florida

CD4⁺CD25⁺ T-cells appear to play a crucial role in regulating the immune response. Therefore, we evaluated the peripheral blood frequency and function of CD4⁺CD25⁺ T-cells in 70 type 1 diabetic patients and 37 healthy individuals. Interestingly, a positive correlation was observed between increasing age and CD4⁺CD25⁺ T-cell frequency in both subject groups. In contrast to previous studies of nonobese diabetic mice and type 1 diabetic patients, similar frequencies of CD4⁺CD25⁺ and CD4⁺CD25^+Bright T-cells were observed in healthy control subjects and type 1 diabetic patients of similar age. There was no difference between type 1 diabetic subjects of recent-onset versus those with established disease in terms of their CD4⁺CD25⁺ or CD4⁺CD25^+BrightT-cell frequency. However, type 1 diabetic patients were markedly defective in their ability to suppress the proliferation of autologous effector T-cells in vitro. This type 1 diabetes-associated defect in suppression was associated with reduced production of interleukin (IL)-2, -interferon, and transforming growth factor-ß, whereas other cytokines including those of adaptive and innate immunity (IL-10, IL-1ß, IL-6, IL-8, IL-12p70, and tumor necrosis factor-) were similar in control subjects and type 1 diabetic patients. These data suggest that age strongly influences the frequency of CD4⁺CD25⁺ T-cells and that function, rather than frequency, may represent the means by which these cells associate with type 1 diabetes in humans.

Abbreviations: APC, allophycocyanin; GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN-, -interferon; IL, interleukin; PE, phycoerythrin; Teff, CD4⁺CD25^– effector T-cell; TGF, transforming growth factor; TNF, tumor necrosis factor; Treg, CD4⁺CD25⁺ regulatory T-cell

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