Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Medarova, Z.
Right arrow Articles by Moore, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Medarova, Z.
Right arrow Articles by Moore, A.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Diabetes 54:1780-1788, 2005
© 2005 by the American Diabetes Association, Inc.

Imaging ß-Cell Death With a Near-Infrared Probe

Zdravka Medarova1, Susan Bonner-Weir2, Myra Lipes2, and Anna Moore1

1 MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts
2 Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts

Evidence exists for an essential role of ß-cell apoptosis in the pathology of type 1 and type 2 diabetes. Current methods for diabetes-associated apoptosis detection, however, suffer the drawbacks of relying on in situ–based strategies. In this study, we attempted to measure, both in vitro and ex vivo, levels of ß-cell apoptosis in diabetic mice using Cy5.5-labeled annexin V. We used streptozotocin-treated BALB/c mice and NOD mice of different ages as models of type 1 diabetes and db/db mice as a model of type 2 diabetes. With annexin V Cy5.5, we established differences in levels of apoptosis between diabetic and control animals. Intravenously administered annexin V Cy5.5 accumulated in pancreata of diabetic mice but not in nondiabetic controls. Furthermore, its localization was specific to apoptotic events within diabetic islets; its selectivity was supported by transferase-mediated dUTP nick-end labeling staining. Because annexin V defines an early marker of apoptosis and the developed probe is suitable for in vivo administration, it may provide a promising tool for real-time identification in intact animals of the earliest stages of diabetes-associated ß-cell death and for tracing the events that characterize the pathology of the disease.

Address correspondence and reprint requests to Anna Moore, PhD, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Room 2301, Building 149, 13th St., Charlestown, MA 02129. E-mail: amoore{at}helix.mgh.harvard.edu

Abbreviations: 7-AAD, 7-amino-actinomycin; 99mTc, technetium-99m; FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; H&E, hematoxylin and eosin; MLDS, multiple low-dose streptozotocin; NIRF, near-infrared fluorescence; SHDS, single high-dose streptozotocin; STZ, streptozotocin; TUNEL, transferase-mediated dUTP nick-end labeling


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2005 by the American Diabetes Association.