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Diabetes 54:2103-2108, 2005
© 2005 by the American Diabetes Association, Inc.

Peptide-Mediated Targeting of the Islets of Langerhans

Kausar N. Samli1, Michael J. McGuire1, Christopher B. Newgard2, Stephen Albert Johnston1, and Kathlynn C. Brown1

1 Center for Biomedical Inventions, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas
2 Sarah W. Stedman Nutrition and Metabolism Center, Departments of Pharmacology and Cancer Biology, Medicine, and Biochemistry, Duke University Medical Center, Durham, North Carolina

Strategies for restoring ß-cell function in diabetic patients would be greatly aided by the ability to target genes, proteins, or small molecules specifically to these cells. Furthermore, the ability to direct imaging agents specifically to ß-cells would facilitate diagnosis and monitoring of disease progression. To isolate ligands that can home to ß-cells in vivo, we have panned a random phage-displayed 20-mer peptide library on freshly isolated rat islets. We have isolated two 20-mer peptides that bind to islets ex vivo. One of these peptides preferentially homes to the islets of Langerhans in a normal rat with clear differentiation between the endocrine and exocrine cells of the pancreas. Furthermore, this peptide does not target ß-cells in a type 2 diabetes animal model, suggesting that the peptide can discriminate between glucose-stimulated insulin secretion–functional and -dysfunctional ß-cells.

Address correspondence and reprint requests to Kathlynn C. Brown, Center for Biomedical Inventions, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9185. E-mail: kathlynn.brown{at}utsouthwestern.edu

Abbreviations: GSIS, glucose-stimulated insulin secretion; HBSS, Hank’s balanced salt solution; RIP, rat islet peptide


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Copyright © 2005 by the American Diabetes Association.