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Diabetes 55:1954-1960, 2006
DOI: 10.2337/db05-1525
© 2006 by the American Diabetes Association
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Comparison of Serum High–Molecular Weight (HMW) Adiponectin With Total Adiponectin Concentrations in Type 2 Diabetic Patients With Coronary Artery Disease Using a Novel Enzyme-Linked Immunosorbent Assay to Detect HMW Adiponectin

Yoshimasa Aso1, Ruriko Yamamoto1, Sadao Wakabayashi1, Toshihiko Uchida2, Kan Takayanagi2, Kohzo Takebayashi1, Takehiko Okuno3, Teruo Inoue4, Koichi Node4, Takashi Tobe5, Toshihiko Inukai1, and Yasuko Nakano5

1 Department of Internal Medicine, Koshigaya Hospital, Dokkyo Medical University, Saitama, Japan
2 Department of Cardiology, Koshigaya Hospital, Dokkyo Medical University, Saitama, Japan
3 Research and Development Division, Fujirebio, Tokyo, Japan
4 Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan
5 Department of Medicinal Information, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan

Address correspondence and reprint requests to Yoshimasa Aso, MD, Department of Internal Medicine, Koshigaya Hospital, Dokkyo Medical University, 2-1-50 Minami-Koshigaya, Koshiagya, Saitama 343-8555, Japan. E-mail: yaso{at}dokkyomed.ac.jp

Abbreviations: CAD, coronary artery disease; CCr, creatinine clearance; ELISA, enzyme-linked immunosorbent assay; HOMA-IR, homeostasis model assessment of insulin resistance; HMW, high molecular weight; LMW, low molecular weight; MMW, medium molecular weight

Adiponectin (Acrp30), an adipocyte-derived protein, exists in serum as a trimer, a hexamer, and a high–molecular weight (HMW) form, including 12–18 subunits. Because HMW adiponectin may be biologically active, we measured it in serum using a novel enzyme-linked immunosorbent assay (ELISA) confirmed by gel filtration chromatography that the ELISA detected mainly adiponectin with 12–18 subunits, and we compared HMW with total adiponectin concentration in patients with type 2 diabetes. We next investigated the relationship between serum HMW and coronary artery disease (CAD) in 280 consecutive type 2 diabetic patients, including 59 patients with angiographically confirmed CAD. Total adiponectin was measured in serum by a commercially available ELISA. Like serum total adiponectin, HMW adiponectin correlated positively with HDL cholesterol and negatively with triglyceride, insulin sensitivity, creatinine clearance, and circulating inflammatory markers. Total and HMW adiponectin were significantly higher in women than in men, as was the HMW-to-total adiponectin ratio. Serum HMW and the HMW-to-total adiponectin ratio were significantly lower in men with than without CAD (P < 0.05, respectively). In women, the ratio, but neither total nor HMW adiponectin, tended to be lower when CAD was present. In conclusion, determination of HMW adiponectin, especially relative to total serum adiponectin, is useful for evaluating CAD in type 2 diabetic patients.


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Copyright © 2006 by the American Diabetes Association.