Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Diabetes 55:2051-2058, 2006
DOI: 10.2337/db06-0175
© 2006 by the American Diabetes Association
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Treebak, J. T.
Right arrow Articles by Wojtaszewski, J. F.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Treebak, J. T.
Right arrow Articles by Wojtaszewski, J. F.P.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

AMPK-Mediated AS160 Phosphorylation in Skeletal Muscle Is Dependent on AMPK Catalytic and Regulatory Subunits

Jonas T. Treebak1, Stephan Glund2, Atul Deshmukh2, Ditte K. Klein1, Yun Chau Long2, Thomas E. Jensen1, Sebastian B. Jørgensen1, Benoit Viollet3, Leif Andersson4, Dietbert Neumann5, Theo Wallimann5, Erik A. Richter1, Alexander V. Chibalin2, Juleen R. Zierath2, and Jørgen F.P. Wojtaszewski1

1 Department of Human Physiology, Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark
2 Department of Molecular Medicine and Surgery, Section Integrative Physiology, Karolinska Institute, Stockholm, Sweden
3 René Descartes University, Institute Cochin, Paris, France
4 Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala Biomedical Center, Uppsala, Sweden
5 Swiss Federal Institute of Technology, Zurich, Switzerland

Address correspondence and reprint requests to Juleen R. Zierath, Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institute, von Eulers väg 4, 4th Floor, S-171 77 Stockholm, Sweden. E-mail: juleen.zierath{at}ki.se

Abbreviations: ACC, acetyl-CoA carboxylase; AICAR, 5-aminoimidazole-4-carboxamide 1 ß-D-ribonucleoside; AMPK, AMP-activated protein kinase; CaMKK, calmodulin-dependent protein kinase kinase; EDL, extensor digitorum longus; GST, glutathione S-transferase; PAS, phospho-Akt substrate; TSC, tuberous sclerosis complex

AMP-activated protein kinase (AMPK) is a heterotrimeric protein that regulates glucose transport mediated by cellular stress or pharmacological agonists such as 5-aminoimidazole-4-carboxamide 1 ß-D-ribonucleoside (AICAR). AS160, a Rab GTPase-activating protein, provides a mechanism linking AMPK signaling to glucose uptake. We show that AICAR increases AMPK, acetyl-CoA carboxylase, and AS160 phosphorylation by insulin-independent mechanisms in isolated skeletal muscle. Recombinant AMPK heterotrimeric complexes ({alpha}1ß1{gamma}1 and {alpha}2ß2{gamma}1) phosphorylate AS160 in a cell-free assay. In mice deficient in AMPK signaling ({alpha}2 AMPK knockout [KO], {alpha}2 AMPK kinase dead [KD], and {gamma}3 AMPK KO), AICAR effects on AS160 phosphorylation were severely blunted, highlighting that complexes containing {alpha}2 and {gamma}3 are necessary for AICAR-stimulated AS160 phosphorylation in intact skeletal muscle. Contraction-mediated AS160 phosphorylation was also impaired in {alpha}2 AMPK KO and KD but not {gamma}3 AMPK KO mice. Our results implicate AS160 as a downstream target of AMPK.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DiabetesHome page
K. Hojlund, D. Glintborg, N. R. Andersen, J. B. Birk, J. T. Treebak, C. Frosig, H. Beck-Nielsen, and J. F.P. Wojtaszewski
Impaired Insulin-Stimulated Phosphorylation of Akt and AS160 in Skeletal Muscle of Women With Polycystic Ovary Syndrome Is Reversed by Pioglitazone Treatment
Diabetes, February 1, 2008; 57(2): 357 - 366.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
H. F. Kramer, E. B. Taylor, C. A. Witczak, N. Fujii, M. F. Hirshman, and L. J. Goodyear
Calmodulin-Binding Domain of AS160 Regulates Contraction- but Not Insulin-Stimulated Glucose Uptake in Skeletal Muscle
Diabetes, December 1, 2007; 56(12): 2854 - 2862.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. Frosig, A. J. Rose, J. T. Treebak, B. Kiens, E. A. Richter, and J. F.P. Wojtaszewski
Effects of Endurance Exercise Training on Insulin Signaling in Human Skeletal Muscle: Interactions at the Level of Phosphatidylinositol 3-Kinase, Akt, and AS160
Diabetes, August 1, 2007; 56(8): 2093 - 2102.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Tzatsos and P. N. Tsichlis
Energy Depletion Inhibits Phosphatidylinositol 3-Kinase/Akt Signaling and Induces Apoptosis via AMP-activated Protein Kinase-dependent Phosphorylation of IRS-1 at Ser-794
J. Biol. Chem., June 22, 2007; 282(25): 18069 - 18082.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. F. Howlett, K. Sakamoto, A. Garnham, D. Cameron-Smith, and M. Hargreaves
Resistance Exercise and Insulin Regulate AS160 and Interaction With 14-3-3 in Human Skeletal Muscle
Diabetes, June 1, 2007; 56(6): 1608 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. A. Witczak, N. Fujii, M. F. Hirshman, and L. J. Goodyear
Ca2+/Calmodulin-Dependent Protein Kinase Kinase-{alpha} Regulates Skeletal Muscle Glucose Uptake Independent of AMP-Activated Protein Kinase and Akt Activation
Diabetes, May 1, 2007; 56(5): 1403 - 1409.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. B. Arias, J. Kim, K. Funai, and G. D. Cartee
Prior exercise increases phosphorylation of Akt substrate of 160 kDa (AS160) in rat skeletal muscle
Am J Physiol Endocrinol Metab, April 1, 2007; 292(4): E1191 - E1200.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. A. B. Davey, P. B. Garlick, A. Warley, and R. Southworth
Immunogold labeling study of the distribution of GLUT-1 and GLUT-4 in cardiac tissue following stimulation by insulin or ischemia
Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H2009 - H2019.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
A. Sriwijitkamol, D. K. Coletta, E. Wajcberg, G. B. Balbontin, S. M. Reyna, J. Barrientes, P. A. Eagan, C. P. Jenkinson, E. Cersosimo, R. A. DeFronzo, et al.
Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 Diabetes: A Time-Course and Dose-Response Study
Diabetes, March 1, 2007; 56(3): 836 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. T. Treebak, J. B. Birk, A. J. Rose, B. Kiens, E. A. Richter, and J. F. P. Wojtaszewski
AS160 phosphorylation is associated with activation of {alpha}2beta2{gamma}1- but not {alpha}2beta2{gamma}3-AMPK trimeric complex in skeletal muscle during exercise in humans
Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E715 - E722.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. C. Towler and D. G. Hardie
AMP-Activated Protein Kinase in Metabolic Control and Insulin Signaling
Circ. Res., February 16, 2007; 100(3): 328 - 341.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
F. S.L. Thong, P. J. Bilan, and A. Klip
The Rab GTPase-Activating Protein AS160 Integrates Akt, Protein Kinase C, and AMP-Activated Protein Kinase Signals Regulating GLUT4 Traffic
Diabetes, February 1, 2007; 56(2): 414 - 423.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2006 by the American Diabetes Association.