HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Online-Only Appendix All Versions of this Article: db06-1165v1 56/1/3    most recent Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chase, L. G. Articles by Verfaillie, C. M. Search for Related Content PubMed PubMed Citation Articles by Chase, L. G. Articles by Verfaillie, C. M. Social Bookmarking                What's this?

Islet-Derived Fibroblast-Like Cells Are Not Derived via Epithelial-Mesenchymal Transition From Pdx-1 or Insulin-Positive Cells

From the Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota

Address correspondence and reprint requests to Catherine M. Verfaillie Stem Cell Institute, University of Minnesota, McGuire Translational Research Facility, 2001 6th St. SE, mail code 2873, Minneapolis, MN 55455. E-mail: verfa001{at}umn.edu

Abbreviations: BM-MSC, bone marrow mesenchymal stem cell; CAAGS, chicken ß-actin promoter/cytomegalovirus enhancer; EMT, epithelial-mesenchymal transition; FACS, fluorescence-activated cell sorter; GFP, green fluorescent protein; MSC, mesenchymal stem cell; qPCR, quantitative PCR

As recent studies suggest that newly formed pancreatic ß-cells are a result of self-duplication rather than stem cell differentiation, in vitro expansion of ß-cells presents a potential mechanism by which to increase available donor tissue for cell-based diabetes therapies. Although most studies have found that ß-cells are resilient to substantial in vitro expansion, recent studies have suggested that it is possible to expand these cells through a process referred to as epithelial-mesenchymal transition (EMT). To further substantiate such an expansion mechanism, we used recombination-based genetic lineage tracing to determine the origin of proliferating fibroblast-like cells from cultured pancreatic islets in vitro. We demonstrate, using two culture methods, that EMT does not underlie the appearance of fibroblast-like cells in mouse islet cultures but that fibroblast-like cells appear to represent mesenchymal stem cell (MSC)-like cells akin to MSCs isolated from bone marrow.

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Bar, H. A. Russ, S. Knoller, L. Ouziel-Yahalom, and S. Efrat HES-1 Is Involved in Adaptation of Adult Human {beta}-Cells to Proliferation In Vitro Diabetes, September 1, 2008; 57(9): 2413 - 2420. [Abstract] [Full Text] [PDF]

				N. Billestrup and T. Otonkoski Dedifferentiation for Replication of Human {beta}-Cells: A Division Between Mice and Men? Diabetes, June 1, 2008; 57(6): 1457 - 1458. [Full Text] [PDF]

				H. A. Russ, Y. Bar, P. Ravassard, and S. Efrat In Vitro Proliferation of Cells Derived From Adult Human {beta}-Cells Revealed By Cell-Lineage Tracing Diabetes, June 1, 2008; 57(6): 1575 - 1583. [Abstract] [Full Text] [PDF]

				Y. Dor and B. Z. Stanger Regeneration in Liver and Pancreas: Time to Cut the Umbilical Cord? Sci. Signal., November 27, 2007; 2007(414): pe66 - pe66. [Abstract] [Full Text] [PDF]